Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,524,914	G→T	L33M (CTG→ATG)	yjhH ←	KpLE2 phage‑like element; putative 2‑dehydro‑3‑deoxy‑D‑pentonate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,524,914	0	G	T	100.0%	91.1 / NA	26	L33M (CTG→ATG)	yjhH	KpLE2 phage‑like element; putative 2‑dehydro‑3‑deoxy‑D‑pentonate aldolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/15);  total (11/15)

GGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAAT  >  NC_000913/4524842‑4524989                                                                         |                                                                            gggCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCATGAAGTCgg                                                                     >  2:867488/1‑81 (MQ=255) gggCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCATGAAGTCgg                                                                     >  2:867481/1‑81 (MQ=255)      gTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACt                                                                >  1:867483/1‑81 (MQ=255)                ggCTAAATTCACCACCGGTACCCAGATAAAGCAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCAtt                                                       <  2:867443/80‑1 (MQ=255)                ggCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCAtt                                                       <  1:867446/80‑1 (MQ=255)                ggCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCAtt                                                       <  1:867445/80‑1 (MQ=255)                     aaTTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCt                                                   <  1:867447/79‑1 (MQ=255)                      aTTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCtttt                                                <  2:867447/81‑1 (MQ=255)                          accaccGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATc                                            <  1:867449/81‑1 (MQ=255)                             accGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAg                                         >  1:867494/1‑81 (MQ=255)                              ccGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAgg                                        <  1:867450/81‑1 (MQ=255)                                 gTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGtt                                     <  1:867451/81‑1 (MQ=255)                                  tACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTc                                    <  2:867451/81‑1 (MQ=255)                                   aCCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTcc                                   >  1:867497/1‑81 (MQ=255)                                     ccAGATAAAACAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGt                                 <  2:867452/81‑1 (MQ=255)                                        gATAAAACAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCAc                              <  1:867454/81‑1 (MQ=255)                                         aTAAAACAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCAc                              >  1:867490/1‑80 (MQ=255)                                            aaaCAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGAt                           <  2:867455/80‑1 (MQ=255)                                            aaaCAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATg                          <  1:867455/81‑1 (MQ=255)                                              aCAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGaa                        <  2:867456/81‑1 (MQ=255)                                               cAGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGaaa                       >  1:867486/1‑81 (MQ=255)                                                aGCCCGTCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAAc                      >  2:867498/1‑81 (MQ=255)                                                      tCGACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTg                >  2:867492/1‑81 (MQ=255)                                                        gACCCCTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGa              >  1:867495/1‑81 (MQ=255)                                                          ccccTTTATTAATCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATa            >  2:867491/1‑81 (MQ=255)                                                                     aTCATGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGaat  <  2:867457/80‑1 (MQ=255)                                                                         |                                                                            GGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAAT  >  NC_000913/4524842‑4524989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑