Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,531,506	A→C	V49G (GTC→GGC)	sgcX ←	KpLE2 phage‑like element; putative endoglucanase with Zn‑dependent exopeptidase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,531,506	0	A	C	100.0%	154.3 / NA	44	V49G (GTC→GGC)	sgcX	KpLE2 phage‑like element; putative endoglucanase with Zn‑dependent exopeptidase domain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/23);  total (21/23)

ATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAG  >  NC_000913/4531431‑4531581                                                                            |                                                                            aTAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACa                                                                        <  2:868949/81‑1 (MQ=255)  tAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACAt                                                                       <  2:868950/81‑1 (MQ=255)  tAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACAt                                                                       >  1:869022/1‑81 (MQ=255)    aaaCCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACAtt                                                                      <  2:868951/80‑1 (MQ=255)        cGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTccc                                                                   >  2:869003/1‑79 (MQ=255)        cGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCaa                                                                 <  1:868954/81‑1 (MQ=255)        cGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCaa                                                                 <  1:868953/81‑1 (MQ=255)          aCTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGc                                                               >  1:869012/1‑81 (MQ=255)          aCTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGc                                                               >  2:868995/1‑81 (MQ=255)           cTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGcc                                                              <  1:868955/81‑1 (MQ=255)            ttCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCt                                                             >  1:869025/1‑81 (MQ=255)                    tatGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGc                                                      >  1:869008/1‑80 (MQ=255)                      tGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAg                                                   <  2:868956/81‑1 (MQ=255)                      tGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAg                                                   <  1:868956/81‑1 (MQ=255)                        cgcAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGAc                                                 >  2:869004/1‑81 (MQ=255)                        cgcAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGAc                                                 <  1:868958/81‑1 (MQ=255)                        cgcAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGAc                                                 <  1:868959/81‑1 (MQ=255)                          cAAAAATCATCAGTCGCAGCGCATCGGGTTTGTCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACct                                               <  2:868960/81‑1 (MQ=255)                          cAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACct                                               <  2:868959/81‑1 (MQ=255)                          cAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACct                                               >  1:869011/1‑81 (MQ=255)                          cAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACct                                               <  1:868962/81‑1 (MQ=255)                             aaaTCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCtt                                            >  1:869027/1‑81 (MQ=255)                              aaTCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCttt                                           >  1:869015/1‑81 (MQ=255)                               atcatcAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCtttt                                          <  1:868963/81‑1 (MQ=255)                               atcatcAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCtttt                                          <  1:868964/81‑1 (MQ=255)                                  atcaGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGcc                                       >  2:869012/1‑81 (MQ=255)                                    caGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCt                                      >  1:869007/1‑80 (MQ=255)                                      gTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGAc                                   <  2:868964/81‑1 (MQ=255)                                         gCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGcc                                >  2:869025/1‑81 (MQ=255)                                          cAGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCt                               <  2:868965/81‑1 (MQ=255)                                           aGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCt                               >  1:869021/1‑80 (MQ=255)                                           aGCGCATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCCTTTGCCTGACGCCTg                              <  1:868967/81‑1 (MQ=255)                                            gcgcATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTg                              >  1:869010/1‑80 (MQ=255)                                            gcgcATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTg                              >  2:869023/1‑80 (MQ=255)                                            gcgcATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTg                              >  2:869017/1‑80 (MQ=255)                                              gcATCGGGTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAAt                           >  1:869014/1‑81 (MQ=255)                                               cATCGGGTTTATCACTACCGTAGCGCGCGCTGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAAtt                          <  2:868967/81‑1 (MQ=255)                                                   gggTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGc                       <  2:868968/80‑1 (MQ=255)                                                    ggTTTATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGca                      >  1:869016/1‑80 (MQ=255)                                                      tttATCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAg                   >  2:868996/1‑81 (MQ=255)                                                          tCACTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCAt                <  2:868969/80‑1 (MQ=255)                                                            aCTACCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGAc             <  1:868971/81‑1 (MQ=255)                                                               aCCGTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGt           >  2:869029/1‑80 (MQ=255)                                                                  gTAGCGCGCGCCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGc       <  1:868972/81‑1 (MQ=255)                                                                       gcgcgcCGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAg  <  1:868973/81‑1 (MQ=255)                                                                            |                                                                            ATAAAACCGACTTCATCCATATGCGCAAAAATCATCAGTCGCAGCGCATCGGGTTTATCACTACCGTAGCGCGCGACGACATTCCCAAGCCTGTCTCGCCAGACCTCTTTTGCCTGACGCCTGAATTCGCACAGCATGACGTCAGCGACAG  >  NC_000913/4531431‑4531581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑