Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,548,591	A→G	T103A (ACC→GCC)	fimG →	type 1 fimbriae minor subunit FimG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,548,591	0	A	G	100.0%	123.5 / NA	36	T103A (ACC→GCC)	fimG	type 1 fimbriae minor subunit FimG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/20);  total (16/20)

GTCCGGTGGGAACGTCGAGGGTCACTGCCAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGACCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAACACATTGAATACTGGCGCAACCAAAACAGTTCA  >  NC_000913/4548514‑4548667                                                                              |                                                                             gTCCGGTGGGAACGTCGAGGGTCACTGCCAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGcc                                                                            <  2:872285/80‑1 (MQ=255) gTCCGGTGGGAACGTCGAGGGTCACTGCCAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGcc                                                                            <  2:872284/80‑1 (MQ=255)    cGGTGGGAACGTCGAGGGTCACTGCCAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCcgcg                                                                         <  1:872287/80‑1 (MQ=255)    cGGTGGGAACGTCGAGGGTCACTGCCAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCcgcg                                                                         >  2:872331/1‑80 (MQ=255)    cGGTGGGAACGTCGAGGGTCACTGCCAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCgcgc                                                                        <  2:872287/81‑1 (MQ=255)         ggAACGTCGAGGGTCACTGCCAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAAc                                                                   <  2:872288/81‑1 (MQ=255)            aCGTCGAGGGTCACTGCCAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATc                                                                >  2:872327/1‑81 (MQ=255)              gTCGAGGGTCACTGCCAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATc                                                                >  1:872317/1‑79 (MQ=255)              gTCGAGGGTCACTGCCAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATc                                                                >  2:872329/1‑79 (MQ=255)               tCGAGGGTCACTGCCAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAg                                                             <  2:872289/81‑1 (MQ=255)                cGAGGGTCACTGCCAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGt                                                            >  2:872317/1‑81 (MQ=255)                cGAGGGTCACTGCCAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGt                                                            >  2:872332/1‑81 (MQ=255)                  aGGGTCACTGCCAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTa                                                          <  2:872290/81‑1 (MQ=255)                           gCCAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAg                                                 >  2:872339/1‑81 (MQ=255)                            ccAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACa                                                  <  1:872292/79‑1 (MQ=255)                               gCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATg                                             <  2:872292/81‑1 (MQ=255)                                   cAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAg                                         >  2:872342/1‑81 (MQ=255)                                       ggggCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTgg                                      >  1:872326/1‑80 (MQ=255)                                        gggCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGc                                     >  2:872328/1‑80 (MQ=255)                                        gggCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGc                                     >  2:872326/1‑80 (MQ=255)                                          gCAGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAac                                  <  1:872294/81‑1 (MQ=255)                                            aGCCGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAacac                                <  2:872294/81‑1 (MQ=255)                                               cGACAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAACACAtt                             <  1:872296/81‑1 (MQ=255)                                                 aCAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAACACATTg                            >  1:872339/1‑80 (MQ=255)                                                 aCAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAACACATTg                            >  2:872347/1‑80 (MQ=255)                                                 aCAGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAACACATTGa                           <  2:872296/81‑1 (MQ=255)                                                   aGTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAACACATTGAAt                         <  2:872297/81‑1 (MQ=255)                                                    gTACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAACACATTGAATa                        >  2:872330/1‑81 (MQ=255)                                                     tACCGGATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAACACATTGAATAc                       <  1:872299/81‑1 (MQ=255)                                                          gATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAACACATTGAATACTGgcg                  >  1:872345/1‑81 (MQ=255)                                                          gATATTATAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAACACATTGAATACTGgcg                  <  1:872300/81‑1 (MQ=255)                                                              ttatAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAACACATTGAATACTGGCGCaa               <  2:872301/80‑1 (MQ=255)                                                              ttatAAAAACCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAACACATTGAATACTGGCGCaa               <  1:872301/80‑1 (MQ=255)                                                                   aaaaCCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAACACATTGAATACTGGCGCAACCaaaa         <  2:872302/81‑1 (MQ=255)                                                                    aaaCCAGGGGGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAACACATTGAATACTGGCGCAACCaaaa         >  2:872345/1‑80 (MQ=255)                                                                          ggggGCCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAACACATTGAATACTGGCGCAACCAAAACAGTTCa  <  2:872303/81‑1 (MQ=255)                                                                              |                                                                             GTCCGGTGGGAACGTCGAGGGTCACTGCCAGCTTCAGCGGGGCAGCCGACAGTACCGGATATTATAAAAACCAGGGGACCGCGCAAAACATCCAGTTAGAGCTACAGGATGACAGTGGCAACACATTGAATACTGGCGCAACCAAAACAGTTCA  >  NC_000913/4548514‑4548667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑