Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,601,522	T→C	intergenic (‑5/+102)	dnaT ← / ← yjjB	primosomal protein DnaT/putative succinate exporter YjjB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,601,522	0	T	C	100.0%	110.0 / NA	32	intergenic (‑5/+102)	dnaT/yjjB	primosomal protein DnaT/putative succinate exporter YjjB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/17);  total (15/17)

CCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACACGGAGGAGAGGAGGCTGT  >  NC_000913/4601445‑4601593                                                                              |                                                                        ccAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGctt                                                                       <  2:883753/80‑1 (MQ=255)  cAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTtct                                                                     <  2:883754/81‑1 (MQ=255)     aaCGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCa                                                                   >  2:883797/1‑80 (MQ=255)        ggTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAata                                                               <  1:883756/81‑1 (MQ=255)        ggTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAata                                                               >  2:883809/1‑81 (MQ=255)              gTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGaa                                                          <  2:883756/80‑1 (MQ=255)               tGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACg                                                        <  1:883758/81‑1 (MQ=255)                 aTCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGAt                                                      <  2:883758/81‑1 (MQ=255)                  tCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGATg                                                     <  1:883760/81‑1 (MQ=255)                  tCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGATg                                                     <  1:883761/81‑1 (MQ=255)                   cGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGATg                                                     >  2:883811/1‑80 (MQ=255)                     tgtACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAAc                                                  >  2:883801/1‑81 (MQ=255)                     tgtACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAAc                                                  <  1:883762/81‑1 (MQ=255)                     tgtACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAAc                                                  <  1:883763/81‑1 (MQ=255)                      gtACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAAc                                                  >  2:883802/1‑80 (MQ=255)                           aGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAg                                            >  1:883818/1‑81 (MQ=255)                                gTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAgg                                           >  2:883808/1‑77 (MQ=255)                                 tCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAGGGAGAt                                      <  1:883764/81‑1 (MQ=255)                                 tCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAGGGAGAt                                      <  2:883764/81‑1 (MQ=255)                                          acgacgTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTtat                             <  2:883765/81‑1 (MQ=255)                                          acgacgTCCGGGGTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTtat                             <  2:883766/81‑1 (MQ=255)                                                     ggTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACACg                  >  1:883825/1‑81 (MQ=255)                                                     ggTCAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACACg                  >  1:883820/1‑81 (MQ=255)                                                      gTCAAAGCTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACACg                  >  1:883811/1‑80 (MQ=255)                                                        cAAAACTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACACggag               >  2:883804/1‑81 (MQ=255)                                                         aaaaCTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACACggagg              <  1:883768/81‑1 (MQ=255)                                                         aaaaCTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACACggag               >  1:883813/1‑80 (MQ=255)                                                           aaCTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACACGgaggag            <  1:883769/81‑1 (MQ=255)                                                            aCTCTGGAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACACggagga             >  2:883820/1‑79 (MQ=255)                                                                  gAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACACGGAGGAGaggagg      <  1:883770/80‑1 (MQ=255)                                                                  gAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACACGGAGGAGAGGAGGc     <  1:883771/81‑1 (MQ=255)                                                                  gAAGACATACTGCTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACACGGAGGAGAGGAGGc     >  2:883832/1‑81 (MQ=255)                                                                     gACATACTGCTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACACGGAGGAGAGGAGGCTGt  >  2:883827/1‑81 (MQ=255)                                                                              |                                                                        CCAGAACGGTTTGGTGATCGTGTACCAGGGCGTCAATACCAACGACGTCCGGGGTCAAAACTCTGGAAGACATACTGTTTCTCAAATATGGAACGATGAACGAGAAGGGAGATAACTTTTATAGTAACACACGGAGGAGAGGAGGCTGT  >  NC_000913/4601445‑4601593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑