Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,607,016	A→G	S229P (TCT→CCT)	rsmC ←	16S rRNA m(2)G1207 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,607,016	0	A	G	100.0%	125.2 / NA	37	S229P (TCT→CCT)	rsmC	16S rRNA m(2)G1207 methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/25);  total (12/25)

GACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCC  >  NC_000913/4606939‑4607091                                                                              |                                                                            gACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGAc                                                                          <  1:884877/81‑1 (MQ=255)        cTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGca                                                                    >  1:884938/1‑80 (MQ=255)            cAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAgg                                                               >  2:884932/1‑81 (MQ=255)              aaGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGt                                                              >  2:884934/1‑80 (MQ=255)                gACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGt                                                              >  2:884923/1‑78 (MQ=255)                gACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTgag                                                           <  2:884878/81‑1 (MQ=255)                gACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTgag                                                           <  2:884877/81‑1 (MQ=255)                  cTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGAc                                                         <  1:884880/81‑1 (MQ=255)                    tCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACg                                                        >  2:884925/1‑80 (MQ=255)                     cACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGa                                                       <  1:884881/80‑1 (MQ=255)                      aCCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAAt                                                     >  1:884904/1‑81 (MQ=255)                           cAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAAtttt                                                  <  2:884882/79‑1 (MQ=255)                           cAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTc                                                 <  2:884881/80‑1 (MQ=255)                           cAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTc                                                 <  2:884883/80‑1 (MQ=255)                           cAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTc                                                 <  2:884884/80‑1 (MQ=255)                            aaCACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCg                                                <  2:884885/80‑1 (MQ=255)                              cacCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCAGCg                                             <  2:884886/81‑1 (MQ=255)                               acCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGa                                            <  2:884887/81‑1 (MQ=255)                                      gCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGgcgc                                     <  1:884890/81‑1 (MQ=255)                                      gCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGgcgc                                     <  2:884888/81‑1 (MQ=255)                                      gCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGgcgc                                     <  2:884890/81‑1 (MQ=255)                                        cGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGgcgcgc                                   <  1:884892/81‑1 (MQ=255)                                           aaGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCa                                  >  1:884940/1‑79 (MQ=255)                                            aGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAg                               <  2:884892/81‑1 (MQ=255)                                              tgtTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCGCAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGc                             <  1:884894/81‑1 (MQ=255)                                              tgtTGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAgg                              >  1:884930/1‑80 (MQ=255)                                                ttGCGCGGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCa                            <  2:884894/80‑1 (MQ=255)                                                    gcgGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTg                        >  2:884940/1‑80 (MQ=255)                                                    gcgGCTGGCTTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGa                       <  1:884896/81‑1 (MQ=255)                                                      ggctggctTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGaaa                     <  1:884897/81‑1 (MQ=255)                                                      ggctggctTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGa                       >  1:884929/1‑79 (MQ=255)                                                           gctTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCa                  <  1:884898/79‑1 (MQ=255)                                                           gctTCTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACg                <  2:884898/81‑1 (MQ=255)                                                               cTACCGCCGGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCcg            >  2:884942/1‑81 (MQ=255)                                                                    gccgGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCca       >  1:884934/1‑81 (MQ=255)                                                                     ccgGGGCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCcac      <  2:884899/81‑1 (MQ=255)                                                                         ggCAGGGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGcc  <  2:884900/81‑1 (MQ=255)                                                                              |                                                                            GACGTTGCTGGCAAAGACTTCACCTTCAACACCGTTGGCCGCAAGTGTTGCGCGGCTGGCTTCTACCGCCGGGGCAGAGACATCGCACAAGGTGAGACGAATTTTCGGCGAATGGCGCGCAAAGGCAACTGAAAGCACGCCCGCGCCACAGCC  >  NC_000913/4606939‑4607091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑