Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,175	A→G	intergenic (+55/‑3)	creC → / → creD	sensory histidine kinase CreC/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,175	0	A	G	100.0%	90.5 / NA	27	intergenic (+55/‑3)	creC/creD	sensory histidine kinase CreC/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/18);  total (9/18)

GACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCC  >  NC_000913/4638098‑4638245                                                                              |                                                                       gACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTa                                                                     <  1:892252/81‑1 (MQ=255)   cTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATg                                                                   >  2:892310/1‑81 (MQ=255)   cTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATg                                                                   <  2:892252/81‑1 (MQ=255)      cACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGtt                                                                 <  1:892254/80‑1 (MQ=255)       aCCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTg                                                                <  1:892255/80‑1 (MQ=255)       aCCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTg                                                                <  1:892256/80‑1 (MQ=255)               ttCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATccccc                                                       >  2:892298/1‑81 (MQ=255)                 cacaTAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCt                                                     >  2:892294/1‑81 (MQ=255)                  acaTAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCt                                                     <  1:892257/80‑1 (MQ=255)                   caTAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGt                                                   <  1:892258/81‑1 (MQ=255)                       gCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCt                                                <  1:892259/80‑1 (MQ=255)                        cTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTgg                                              <  2:892259/81‑1 (MQ=255)                              aTTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAAt                                         >  1:892309/1‑80 (MQ=255)                               ttcttcCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGa                                       <  1:892261/81‑1 (MQ=255)                                 cttcCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGAc                                      >  2:892305/1‑80 (MQ=255)                                     ccACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGcc                                 <  2:892261/81‑1 (MQ=255)                                        caTAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAGTGACTAGCCTGt                              <  1:892263/81‑1 (MQ=255)                                          tAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGttt                            <  2:892263/81‑1 (MQ=255)                                            gTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTgg                          <  2:892264/81‑1 (MQ=255)                                                  gTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGt                    <  2:892265/81‑1 (MQ=255)                                                    aTCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTAt                  >  1:892315/1‑81 (MQ=255)                                                    aTCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTAt                  >  1:892304/1‑81 (MQ=255)                                                          ctgcCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCt              <  1:892267/79‑1 (MQ=255)                                                             ccATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCtgttgt         >  1:892305/1‑81 (MQ=255)                                                                    aaaGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTcc  <  1:892268/81‑1 (MQ=255)                                                                    aaaGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTcc  <  2:892268/81‑1 (MQ=255)                                                                    aaaGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTcc  >  2:892319/1‑81 (MQ=255)                                                                              |                                                                       GACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCC  >  NC_000913/4638098‑4638245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑