Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,335	C→T	K72K (AAG→AAA)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,335	0	C	T	100.0%	106.8 / NA	32	K72K (AAG→AAA)	araD	L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/17);  total (15/17)

CGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGT  >  NC_000913/66257‑66408                                                                               |                                                                          cgAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGttt                                                                         <  2:14605/81‑1 (MQ=255)           tGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCt                                                               <  2:14606/81‑1 (MQ=255)             cacaATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCtt                                                              >  2:14645/1‑80 (MQ=255)              acaATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTc                                                             >  1:14660/1‑80 (MQ=255)              acaATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCa                                                            <  1:14607/81‑1 (MQ=255)                aaTGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAAc                                                          <  1:14608/81‑1 (MQ=255)                   gccgccAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGCGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCAc                                                       >  1:14652/1‑81 (MQ=255)                   gccgccAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCAc                                                       >  1:14646/1‑81 (MQ=255)                    ccgccAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACt                                                      <  1:14609/81‑1 (MQ=255)                      gccAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTc                                                    <  2:14610/81‑1 (MQ=255)                        cAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCAc                                                  <  2:14611/81‑1 (MQ=255)                         aaTGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCAcc                                                 <  2:14612/81‑1 (MQ=255)                         aaTGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCAcc                                                 <  1:14613/81‑1 (MQ=255)                                  gAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGAt                                        <  2:14614/81‑1 (MQ=255)                                   aaTGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATg                                       >  1:14659/1‑81 (MQ=255)                                   aaTGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATg                                       <  1:14615/81‑1 (MQ=255)                                    aTGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGc                                      >  1:14648/1‑81 (MQ=255)                                        cTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAAc                                  <  1:14616/81‑1 (MQ=255)                                        cTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAAc                                  >  1:14655/1‑81 (MQ=255)                                                  agcCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCata                        >  1:14664/1‑81 (MQ=255)                                                   gcCGGTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCatat                       >  2:14657/1‑81 (MQ=255)                                                      ggTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGt                    >  2:14668/1‑81 (MQ=255)                                                       gTGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTc                   <  1:14617/81‑1 (MQ=255)                                                        tGAGTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGt                    >  2:14662/1‑79 (MQ=255)                                                           gTTGGCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCg               >  2:14675/1‑81 (MQ=255)                                                               gCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTc            <  1:14618/80‑1 (MQ=255)                                                               gCGTGTCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTc            <  1:14619/80‑1 (MQ=255)                                                                    tCGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGAc       <  2:14620/80‑1 (MQ=255)                                                                     cGGAGGAGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGc     >  2:14676/1‑81 (MQ=255)                                                                      ggaggaGGGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCt    <  1:14621/81‑1 (MQ=255)                                                                        aggaggGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTg   >  2:14663/1‑80 (MQ=255)                                                                        aggaggGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGt  <  1:14622/81‑1 (MQ=255)                                                                         ggaggGTTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGt  >  2:14673/1‑80 (MQ=255)                                                                               |                                                                          CGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGT  >  NC_000913/66257‑66408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑