Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	462,215	C→A	L101M (CTG→ATG)	ppiD →	periplasmic folding chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	462,215	0	C	A	100.0%	129.6 / NA	36	L101M (CTG→ATG)	ppiD	periplasmic folding chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/14);  total (22/14)

ACTCCGAGCTGGCAGCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGCTGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAGC  >  NC_000913/462135‑462291                                                                                 |                                                                             aCTCCGAGCTGGCAGCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCgctgc                                                                              >  2:93663/1‑81 (MQ=255)    ccGAGCTGGCAGCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGctgatg                                                                            >  1:93658/1‑80 (MQ=255)     cGAGCTGGCAGCGAACGAGGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATgg                                                                           <  2:93614/80‑1 (MQ=255)      gAGCTGGCAGCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGAt                                                                         >  1:93656/1‑81 (MQ=255)            gCAGCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGt                                                                     <  1:93615/79‑1 (MQ=255)             cAGCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACg                                                                  >  1:93661/1‑81 (MQ=255)              aGCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGc                                                                 <  2:93616/81‑1 (MQ=255)               gCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCa                                                                >  2:93655/1‑81 (MQ=255)                cgaacgaaGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCAc                                                               <  2:93617/81‑1 (MQ=255)                   acgaaGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGt                                                             >  2:93664/1‑80 (MQ=255)                   acgaaGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGt                                                             >  1:93673/1‑80 (MQ=255)                    cgaaGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGt                                                             >  1:93654/1‑79 (MQ=255)                       aGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCt                                                        <  1:93618/81‑1 (MQ=255)                        ggCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCt                                                        >  2:93659/1‑80 (MQ=255)                         gCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCTGa                                                      <  1:93619/81‑1 (MQ=255)                                 aaaaCCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTggg                                               >  1:93651/1‑80 (MQ=255)                                  aaaCCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTa                                             >  1:93675/1‑81 (MQ=255)                                      ccTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAg                                         <  2:93620/81‑1 (MQ=255)                                           gTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGAt                                     >  1:93679/1‑80 (MQ=255)                                           gTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGAt                                     >  1:93677/1‑80 (MQ=255)                                           gTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATg                                    <  2:93621/81‑1 (MQ=255)                                             cAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAg                                  >  1:93667/1‑81 (MQ=255)                                              aaCAGGTGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAg                                  <  2:93622/80‑1 (MQ=255)                                                    tGCTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTa                           <  1:93623/81‑1 (MQ=255)                                                      cTGAATCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTaaa                         >  2:93670/1‑81 (MQ=255)                                                         aaTCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAAc                        <  1:93624/79‑1 (MQ=255)                                                           tCGTCTGATCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGc                    <  1:93625/81‑1 (MQ=255)                                                                  aTCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGAttt               <  1:93626/79‑1 (MQ=255)                                                                   tCGACGAGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTcg            >  1:93680/1‑81 (MQ=255)                                                                    cgacgaGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTcgc           >  1:93666/1‑81 (MQ=255)                                                                    cgacgaGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTcgc           >  2:93643/1‑81 (MQ=255)                                                                     gacgaGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTcgcg          <  2:93627/81‑1 (MQ=255)                                                                     gacgaGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTcgc           >  2:93676/1‑80 (MQ=255)                                                                     gacgaGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTcgc           >  2:93678/1‑80 (MQ=255)                                                                      acgaGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGa         >  1:93674/1‑81 (MQ=255)                                                                        gaGGCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGAcc       >  2:283749/1‑81 (MQ=255)                                                                          ggCGCTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGAccc      >  2:93681/1‑80 (MQ=255)                                                                            cgcTGATGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAg   >  1:93668/1‑81 (MQ=255)                                                                             gctgatGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAGc  <  1:93628/81‑1 (MQ=255)                                                                                 |                                                                             ACTCCGAGCTGGCAGCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGCTGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAGC  >  NC_000913/462135‑462291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑