Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	471,053	G→T	V140F (GTT→TTT)	mdlB →	ABC transporter family protein MdlB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	471,053	0	G	T	100.0%	105.5 / NA	30	V140F (GTT→TTT)	mdlB	ABC transporter family protein MdlB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/9);  total (21/9)

GCGAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATGTTCAGCCTCGACT  >  NC_000913/470976‑471128                                                                              |                                                                            gCGAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACTTTa                                                                          >  2:95363/1‑81 (MQ=255)   gAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACTTTAcc                                                                        >  1:95351/1‑81 (MQ=255)          aTACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTgg                                                                 >  2:95352/1‑81 (MQ=255)                   ccGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTcc                                                        >  1:95378/1‑81 (MQ=255)                    cGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCt                                                       >  2:95360/1‑81 (MQ=255)                     gTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTg                                                      >  1:95370/1‑81 (MQ=255)                     gTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTg                                                      <  1:95327/81‑1 (MQ=255)                      tCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTgc                                                     <  1:95328/81‑1 (MQ=255)                      tCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTgc                                                     >  2:95362/1‑81 (MQ=255)                        gggCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTgcg                                                    >  1:95365/1‑80 (MQ=255)                         ggCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCa                                                  <  1:95329/81‑1 (MQ=255)                          gCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAg                                                 <  1:95330/81‑1 (MQ=255)                            aGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTg                                               >  2:177143/1‑81 (MQ=255)                                   ttCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCt                                        >  1:95366/1‑81 (MQ=255)                                          gTCACTAATGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGgc                                 >  1:95381/1‑81 (MQ=255)                                           tCACTAATGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGgcg                                <  1:95331/81‑1 (MQ=255)                                             aCTAATGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGcgcg                              <  1:95333/81‑1 (MQ=255)                                             aCTAATGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGcgcg                              <  1:95332/81‑1 (MQ=255)                                                 aTGACACTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGc                          >  2:95367/1‑81 (MQ=255)                                                    acacTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCtgg                       >  2:95371/1‑81 (MQ=255)                                                       cTGAAGTGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCtggtgg                    <  2:95334/81‑1 (MQ=255)                                                             tGATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATGt              <  1:95335/81‑1 (MQ=255)                                                              gATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATGtt             >  1:95375/1‑81 (MQ=255)                                                              gATCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATGtt             >  1:95407/1‑81 (MQ=255)                                                                tCCGCGATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATGtt             >  2:95383/1‑79 (MQ=255)                                                                   gcgATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATGTTCAGcc        >  1:95364/1‑81 (MQ=255)                                                                   gcgATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATGTTCAGcc        >  2:95368/1‑81 (MQ=255)                                                                     gATCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATGTTCAGCCTc      >  2:95369/1‑81 (MQ=255)                                                                      aTCTCTACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATGTTCAGCCTc      >  2:95376/1‑80 (MQ=255)                                                                         tctACTTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATGTTCAGCCTCGACt  >  2:95382/1‑81 (MQ=255)                                                                              |                                                                            GCGAGTTTGATACCCAACCCGTCGGGCAGGTGATTTCCCGCGTCACTAATGACACTGAAGTGATCCGCGATCTCTACGTTACCGTAGTGGCAACTGTCCTGCGCAGTGCCGCGCTGGTGGGCGCGATGCTGGTGGCGATGTTCAGCCTCGACT  >  NC_000913/470976‑471128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑