Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	480,275	A→G	L12L (TTA→CTA)	hha ←	hemolysin expression modulating protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	480,275	0	A	G	100.0%	51.7 / NA	16	L12L (TTA→CTA)	hha	hemolysin expression modulating protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/9);  total (7/9)

ATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAAC  >  NC_000913/480206‑480347                                                                      |                                                                         aTTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGttt                                                               >  1:97082/1‑81 (MQ=255) aTTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGttt                                                               <  2:97049/81‑1 (MQ=255)      tatttattTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGt                                                           >  1:97077/1‑80 (MQ=255)            tttttCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGt                                                    <  2:97050/81‑1 (MQ=255)              tttCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGttt                                                  >  1:97083/1‑81 (MQ=255)                    gATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCgg                                             <  1:97051/80‑1 (MQ=255)                       aaCACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACa                                          >  2:97081/1‑80 (MQ=255)                        acacGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATa                                        >  1:97074/1‑81 (MQ=255)                            gCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTc                                    <  2:97052/81‑1 (MQ=255)                                       tCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGt                          <  2:97053/80‑1 (MQ=255)                                       tCAATTGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGt                          <  2:97054/80‑1 (MQ=255)                                           ttGTCTGGCAACGACGTAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGtaa                     <  2:97055/81‑1 (MQ=255)                                                           tAAACGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATa     <  1:97056/81‑1 (MQ=255)                                                            aaaCGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATa     >  2:97080/1‑80 (MQ=255)                                                             aaCGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATaaa   <  2:97057/81‑1 (MQ=255)                                                              aCGCATTAGATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAAc  >  2:97086/1‑81 (MQ=255)                                                                      |                                                                         ATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAAC  >  NC_000913/480206‑480347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑