Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	508,288	A→G	D24G (GAT→GGT)	ybaQ →	putative DNA‑binding transcriptional regulator YbaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	508,288	0	A	G	100.0%	83.9 / NA	25	D24G (GAT→GGT)	ybaQ	putative DNA‑binding transcriptional regulator YbaQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/13);  total (12/13)

ACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCG  >  NC_000913/508215‑508363                                                                          |                                                                            aCCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGaaa                                                                      <  1:102266/81‑1 (MQ=255)   cATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAAt                                                                    >  1:102307/1‑81 (MQ=255)        aaCAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAAt                                                                >  2:102292/1‑80 (MQ=255)                acaaGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAg                                                         >  1:102289/1‑79 (MQ=255)                 caaGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCag                                                      <  2:102267/81‑1 (MQ=255)                  aaGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCa                                                       >  2:102294/1‑79 (MQ=255)                    gAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGt                                                   <  2:102268/81‑1 (MQ=255)                        aCCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTtgct                                               <  1:102269/81‑1 (MQ=255)                         ccGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTtgctg                                              <  2:102270/81‑1 (MQ=255)                          cgacgaCACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTtgctgc                                             <  2:102271/81‑1 (MQ=255)                              gacACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGt                                         <  2:102272/81‑1 (MQ=255)                                     ggAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGt                                  <  1:102273/81‑1 (MQ=255)                                      gagaTATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTa                                 <  2:102274/81‑1 (MQ=255)                                          tatTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAg                             >  1:102297/1‑81 (MQ=255)                                            ttcttcTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCg                           >  1:102296/1‑81 (MQ=255)                                                   ctATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTg                     >  2:102295/1‑80 (MQ=255)                                                      tGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGc                    <  2:102275/78‑1 (MQ=255)                                                        aaTATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTg                <  2:102276/80‑1 (MQ=255)                                                        aaTATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTg                <  2:102277/80‑1 (MQ=255)                                                               ggAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATaa        >  2:102326/1‑81 (MQ=255)                                                                gAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAAt          <  1:102278/78‑1 (MQ=255)                                                                    cGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATc   >  1:102320/1‑81 (MQ=255)                                                                     gCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCg  >  2:102309/1‑81 (MQ=255)                                                                      cTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCg  >  1:102310/1‑80 (MQ=255)                                                                      cTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCg  >  1:102299/1‑80 (MQ=255)                                                                          |                                                                            ACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCG  >  NC_000913/508215‑508363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑