Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	519,900	C→T	L56L (CTC→CTT)	ybbA →	putative ABC transporter ATP‑binding protein YbbA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	519,900	0	C	T	100.0%	89.2 / NA	25	L56L (CTC→CTT)	ybbA	putative ABC transporter ATP‑binding protein YbbA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/16);  total (9/16)

TGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCTGGTGGGACAACCGCTACATAATATGGACGAAGAAGC  >  NC_000913/519824‑519974                                                                             |                                                                           tGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTTGCCg                                                                        >  2:104565/1‑81 (MQ=255)   gTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTTGCCgg                                                                       <  2:104529/80‑1 (MQ=255)   gTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTTGCCg                                                                        <  1:104528/79‑1 (MQ=255)                  gagaCCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTTGCCGGGCTTGATGACGgcagc                                                       <  1:104530/81‑1 (MQ=255)                     aCCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGt                                                    <  1:104531/81‑1 (MQ=255)                       cATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTgg                                                  <  1:104532/81‑1 (MQ=255)                             aCTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGt                                            <  2:104533/81‑1 (MQ=255)                                ggtggGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAg                                         >  1:104574/1‑81 (MQ=255)                                 gtggGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGa                                          <  2:104534/79‑1 (MQ=255)                                      cGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCtggt                                   <  2:104535/81‑1 (MQ=255)                                      cGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCtggt                                   <  2:104536/81‑1 (MQ=255)                                        aGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCtggtgg                                 >  2:104579/1‑81 (MQ=255)                                            gggATCGGGTAAGTCAACCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCTGGTGGGAc                              <  1:104537/80‑1 (MQ=255)                                             ggATCGGGTAAGTCAACCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCTGGTGGGACaa                            >  1:104572/1‑81 (MQ=255)                                             ggATCGGGTAAGTCAACCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCTGGTGGGACaa                            >  1:104573/1‑81 (MQ=255)                                               aTCGGGTAAGTCAACCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCTGGTGGGACAAcc                          <  2:104538/81‑1 (MQ=255)                                                 cGGGTAAGTCAACCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCTGGTGGGACAACCGc                        >  2:104580/1‑81 (MQ=255)                                                          cAACCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCTGGTGGGACAACCGCTACataata               >  2:104575/1‑81 (MQ=255)                                                           aaCCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCTGGTGGGACAACCGCTACataata               <  1:104539/80‑1 (MQ=255)                                                           aaCCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCTGGTGGGACAACCGCTACATAatat              >  2:104583/1‑81 (MQ=255)                                                            aCCTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCTGGTGGGACAACCGCTACATAATATg             >  1:104584/1‑81 (MQ=255)                                                             ccTTGCTGGCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCTGGTGGGACAACCGCTACATAATATgg            <  2:104540/81‑1 (MQ=255)                                                                     gCGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCTGGTGGGACAACCGCTACATAATATGGACgaag      <  1:104541/79‑1 (MQ=255)                                                                      cGATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCTGGTGGGACAACCGCTACATAATATGGACGaagaag   <  1:104542/81‑1 (MQ=255)                                                                       gATCCTTGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCTGGTGGGACAACCGCTACATAATATGGACGAAGAAgc  <  2:104543/81‑1 (MQ=255)                                                                             |                                                                           TGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGCCGGGCTTGATGACGGCAGCAGTGGCGAAGTGAGTCTGGTGGGACAACCGCTACATAATATGGACGAAGAAGC  >  NC_000913/519824‑519974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑