Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	538,029	A→G	I133V (ATT→GTT)	ybbW →	putative allantoin transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	538,029	0	A	G	100.0%	61.8 / NA	19	I133V (ATT→GTT)	ybbW	putative allantoin transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/14);  total (5/14)

ATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCG  >  NC_000913/537959‑538103                                                                       |                                                                           attaAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTgg                                                                  >  1:107883/1‑81 (MQ=255)         gCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCt                                                          <  1:107849/81‑1 (MQ=255)         gCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCt                                                          <  1:107848/81‑1 (MQ=255)             aaTTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGcc                                                      >  2:107888/1‑81 (MQ=255)              aTTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCg                                                     >  2:107894/1‑81 (MQ=255)               ttGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCgg                                                    <  1:107850/81‑1 (MQ=255)                    gccATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGAttt                                               <  2:107851/81‑1 (MQ=255)                      catcatGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGAttttt                                             <  2:107853/81‑1 (MQ=255)                      catcatGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGAttttt                                             <  1:107852/81‑1 (MQ=255)                             tggtttggttTGCAATGTTACTCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACtctc                                      <  2:107854/81‑1 (MQ=255)                                    gtttgCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGgtggtg                               <  2:107855/81‑1 (MQ=255)                                        gCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGAttt                           >  1:107890/1‑81 (MQ=255)                                             gTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCActc                      >  1:107886/1‑81 (MQ=255)                                                 cgcgGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGtt                  <  2:107856/81‑1 (MQ=255)                                                      ggTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGcc             <  1:107857/81‑1 (MQ=255)                                                          aCTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTtc         <  2:107858/81‑1 (MQ=255)                                                              gCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTctct      <  2:107859/80‑1 (MQ=255)                                                                cTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTAcc   <  1:107860/81‑1 (MQ=255)                                                                   cTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCg  <  1:107861/79‑1 (MQ=255)                                                                       |                                                                           ATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCG  >  NC_000913/537959‑538103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑