Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	539,434	G→A	L96L (CTG→CTA)	allB →	allantoinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	539,434	0	G	A	100.0%	72.8 / NA	21	L96L (CTG→CTA)	allB	allantoinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/11);  total (10/11)

GAAGGTTATGAAACCGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTGCCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCGG  >  NC_000913/539357‑539507                                                                              |                                                                          gAAGGTTATGAAACCGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGctacct                                                                        <  1:108073/81‑1 (MQ=255)     gTTATGAAACCGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCaa                                                                    <  2:108074/81‑1 (MQ=255)        aTGAAACCGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACgg                                                                 >  2:108107/1‑81 (MQ=255)              ccGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGAcc                                                           <  2:108075/81‑1 (MQ=255)                  tACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCgcgc                                                       <  1:108076/81‑1 (MQ=255)                  tACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCgcgc                                                       >  1:108098/1‑81 (MQ=255)                           aGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTg                                               >  2:108111/1‑80 (MQ=255)                           aGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTg                                               >  1:108103/1‑80 (MQ=255)                             cGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAAc                                            >  2:108109/1‑81 (MQ=255)                                   aaGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGt                                      <  2:108077/81‑1 (MQ=255)                                    aGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGtt                                     <  1:108078/81‑1 (MQ=255)                                    aGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTAAAGtt                                     <  2:108079/81‑1 (MQ=255)                                        ggtATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGAt                                 >  1:108110/1‑81 (MQ=255)                                          tATCACCACCATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGc                               <  1:108080/81‑1 (MQ=255)                                              accaccATGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGc                            >  2:108105/1‑80 (MQ=255)                                                   caTGATCGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAgg                      >  2:108106/1‑81 (MQ=255)                                                         cGAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCt                >  2:108124/1‑81 (MQ=255)                                                          gAAATGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTg               <  2:108081/81‑1 (MQ=255)                                                             aTGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACt            <  2:108082/81‑1 (MQ=255)                                                              tGCCGCTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTa           <  1:108083/81‑1 (MQ=255)                                                                   cTCAACCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGAt      <  2:108084/81‑1 (MQ=255)                                                                       aCCAGCTACCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCgg  >  2:108115/1‑81 (MQ=255)                                                                              |                                                                          GAAGGTTATGAAACCGGTACTCGCGCAGCGGCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTGCCTGCAACGGTTGACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCGG  >  NC_000913/539357‑539507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑