Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	552,391	A→G	F68F (TTT→TTC)	purK ←	5‑(carboxyamino)imidazole ribonucleotide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	552,391	0	A	G	100.0%	94.8 / NA	28	F68F (TTT→TTC)	purK	5‑(carboxyamino)imidazole ribonucleotide synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/15);  total (13/15)

GGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGC  >  NC_000913/552317‑552464                                                                           |                                                                          ggCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGcc                                                                     >  2:110577/1‑81 (MQ=255)  gCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGCCg                                                                    >  2:110589/1‑81 (MQ=255)     ggTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGCCGGAt                                                                 >  1:110603/1‑81 (MQ=255)      gTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGCCGGATg                                                                >  1:110590/1‑81 (MQ=255)      gTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGCCGGATg                                                                >  1:110602/1‑81 (MQ=255)       tGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGCCGGATgg                                                               <  2:110548/81‑1 (MQ=255)         gAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGCCGGATGgc                                                              <  2:110549/80‑1 (MQ=255)          aGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGCCGGATGgcgc                                                            >  2:110594/1‑81 (MQ=255)            cTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGCCGGATGgcgcgc                                                          >  2:110612/1‑81 (MQ=255)               aTCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGCCGGATGGCGCGCCa                                                        <  2:110550/80‑1 (MQ=255)                    aaaGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGCCGGATGGCGCGCCAGCTcgc                                                  <  1:110551/81‑1 (MQ=255)                        ctgctTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGt                                              <  1:110552/81‑1 (MQ=255)                               tGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCg                                       >  1:110592/1‑81 (MQ=255)                                 aGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCg                                       <  2:110553/79‑1 (MQ=255)                                     aGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTcc                                 <  2:110554/81‑1 (MQ=255)                                     aGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTc                                  <  1:110555/80‑1 (MQ=255)                                          gTCAGCAATAATCGGGAACACATCGCGGTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCa                            <  2:110556/81‑1 (MQ=255)                                              gCAATAATCGGGAACACATCGCGGTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGt                        <  2:110557/81‑1 (MQ=255)                                                     tCGGGAACACATCGCGGTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTAtct                 >  2:110596/1‑81 (MQ=255)                                                        ggAACACATCGCGGTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTCCTATCCCAg              >  2:110609/1‑81 (MQ=255)                                                         gAACACATCGCGGTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGc             <  1:110558/81‑1 (MQ=255)                                                         gAACACATCGCGGTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGc             >  2:110601/1‑81 (MQ=255)                                                          aaCACATCGCGGTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCg            <  2:110559/81‑1 (MQ=255)                                                            cacaTCGCGGTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGt          <  2:871042/81‑1 (MQ=255)                                                               aTCGCGGTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAAt       >  2:110607/1‑81 (MQ=255)                                                                  gcgGTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCAc    <  1:110561/81‑1 (MQ=255)                                                                  gcgGTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCAc    <  1:110560/81‑1 (MQ=255)                                                                    ggTTCACGAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGc  >  1:110598/1‑81 (MQ=255)                                                                           |                                                                          GGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGC  >  NC_000913/552317‑552464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑