Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	560,244	A→G	N183S (AAT→AGT)	sfmD →	putative fimbrial usher protein SfmD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	560,244	0	A	G	100.0%	60.1 / NA	18	N183S (AAT→AGT)	sfmD	putative fimbrial usher protein SfmD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/8);  total (10/8)

TTGAGTAGCGCTCACGGTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAATTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGC  >  NC_000913/560177‑560320                                                                    |                                                                             ttgAGTAGCGCTCACGGTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCAcc                                                                 <  2:111919/81‑1 (MQ=255)   gAGTAGCGCTCACGGTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCgg                                                               >  2:111940/1‑81 (MQ=255)   gAGTAGCGCTCACGGTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCgg                                                               >  1:111950/1‑81 (MQ=255)             cACGGTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAgg                                                      >  1:111942/1‑80 (MQ=255)             cACGGTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAgg                                                      >  1:111948/1‑80 (MQ=255)                 gTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATg                                                 <  1:111920/81‑1 (MQ=255)                     cATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTaa                                             <  1:111921/81‑1 (MQ=255)                         ccGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGa                                          >  1:111944/1‑80 (MQ=255)                          cGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATa                                        <  1:111922/81‑1 (MQ=255)                            ccTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGc                                      >  1:111943/1‑81 (MQ=255)                            ccTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGc                                      <  1:111923/81‑1 (MQ=255)                             cTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGc                                      >  1:111951/1‑80 (MQ=255)                                    gTGGGATGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTAtttttt                              >  2:111941/1‑81 (MQ=255)                                         aTGAAGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTATTTTTTTAGt                          >  1:111947/1‑80 (MQ=255)                                            aaGGTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGCAATGGTAACGATAGCTATTTTTTTAGTGAGc                      >  2:111953/1‑81 (MQ=255)                                               gTATTCCTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTATTTTTTTAGTGAGCTCa                   <  2:111924/81‑1 (MQ=255)                                                     cTGCTTTACTCCTGAGTTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTATTTTTTTAGTGAGCTCAGCGGGa             <  2:111925/81‑1 (MQ=255)                                                                cTGAGTTATAATTTCACCGGTAACAGAGGTAGTGGTAACGATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGc  <  2:111926/81‑1 (MQ=255)                                                                    |                                                                             TTGAGTAGCGCTCACGGTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAATTATAATTTCACCGGTAACAGAGGTAATGGTAACGATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGC  >  NC_000913/560177‑560320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑