Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	568,797	T→C	Y35H (TAT→CAT)	ylcJ →	protein YlcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	568,797	0	T	C	100.0%	50.9 / NA	16	Y35H (TAT→CAT)	ylcJ	protein YlcJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/6);  total (10/6)

TTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCTTTTTAAATAAAAAATAAACAAAAATTATATCCCACCACTAAGGT  >  NC_000913/568728‑568871                                                                      |                                                                           ttATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGCATATTCAAGa                                                                  >  2:113335/1‑80 (MQ=255)  tATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGCATATTCAAg                                                                   <  2:113321/78‑1 (MQ=255)   aTTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGCATATTCAAGATaa                                                               >  1:113339/1‑81 (MQ=255)        ttATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGCATATTCAAGATAATGTGa                                                          >  2:113336/1‑81 (MQ=255)                 cTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGCATATTCAAGATAATGTGACCTTAATTg                                                 >  2:113353/1‑81 (MQ=255)                    ttGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGCATATTCAAGATAATGTGACCTTAATTGTCt                                              >  2:113341/1‑81 (MQ=255)                       tgCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGCATATTCAAGATAATGTGACCTTAATTGTCtttt                                           >  1:113333/1‑81 (MQ=255)                         cTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGCATATTCAAGATAATGTGACCTTAATTGTCTTTTta                                         <  1:113322/81‑1 (MQ=255)                                     tGGCGTAAGAAAATTGCAATACGATTATTGTTGCATATTCAAGATAATGTGACCTTAATTGTCTTTTTaaataaaaaataa                             <  2:113323/81‑1 (MQ=255)                                      ggCGTAAGAAAATTGCAATACGATTATTGTTGCATATTCAAGATAATGTGACCTTAATTGTCTTTTTaaataaaaaataaa                            >  2:113344/1‑81 (MQ=255)                                            aGAAAATTGCAATACGATTATTGTTGCATATTCAAGATAATGTGACCTTAATTGTCTTTTTAAATAAAAAATAAACaaaa                       <  2:113324/80‑1 (MQ=255)                                              aaaaTTGCAATACGATTATTGTTGCATATTCAAGATAATGTGACCTTAATTGTCTTTTTAAATAAAAAATAAACAAAAAt                     >  1:113337/1‑80 (MQ=255)                                               aaaTTGCAATACGATTATTGTTGCATATTCAAGATAATGTGACCTTAATTGTCTTTTTAAATAAAAAATAAACAAAAATta                   >  2:113338/1‑81 (MQ=255)                                                     cAATACGATTATTGTTGCATATTCAAGATAATGTGACCTTAATTGTCTTTTTAAATAAAAAATAAACAAAAATTATATcc              <  2:113325/80‑1 (MQ=255)                                                               attGTTGCATATTCAAGATAATGTGACCTTAATTGTCTTTTTAAATAAAAAATAAACAAAAATTATATCCCACCACTAAgg   >  1:113342/1‑81 (MQ=255)                                                                ttgttgCATATTCAAGATAATGTGACCTTAATTGTCTTTTTAAATAAAAAATAAACAAAAATTATATCCCACCACTAAGGt  <  2:113326/81‑1 (MQ=255)                                                                      |                                                                           TTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCTTTTTAAATAAAAAATAAACAAAAATTATATCCCACCACTAAGGT  >  NC_000913/568728‑568871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑