Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	582,267	G→A	pseudogene (540/655 nt)	ybcY ←	DLP12 prophage; putative SAM‑dependent methyltransferase YbcY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	582,267	0	G	A	100.0%	101.7 / NA	29	pseudogene (540/655 nt)	ybcY	DLP12 prophage; putative SAM‑dependent methyltransferase YbcY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/13);  total (16/13)

CTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGACCGAAGCTATTGTGCACAACTCCATCGCC  >  NC_000913/582190‑582337                                                                              |                                                                       cTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTaaaa                                                                     <  1:115283/81‑1 (MQ=255)    gAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAg                                                                   <  2:115284/80‑1 (MQ=255)       ccTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGc                                                               >  1:115317/1‑81 (MQ=255)        cTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGcc                                                              <  1:115286/81‑1 (MQ=255)        cTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGcc                                                              <  2:115285/81‑1 (MQ=255)                    aTTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTa                                                  <  1:115287/81‑1 (MQ=255)                       ctcGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAAt                                               >  2:115327/1‑81 (MQ=255)                          gAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATgcg                                            >  2:115316/1‑81 (MQ=255)                          gAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATgc                                             <  2:115288/80‑1 (MQ=255)                               gCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCAt                                         >  1:115306/1‑79 (MQ=255)                                   tGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGttt                                   >  1:115313/1‑81 (MQ=255)                                     agagTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGttttt                                 >  2:115326/1‑81 (MQ=255)                                     agagTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGttttt                                 >  1:115325/1‑81 (MQ=255)                                     agagTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGttttt                                 <  2:115290/81‑1 (MQ=255)                                     agagTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGtttt                                  <  2:115289/80‑1 (MQ=255)                                       agTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGa                               >  1:115311/1‑81 (MQ=255)                                         tataTGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGAcc                             <  1:115291/81‑1 (MQ=255)                                           tatGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGACCg                            >  2:115312/1‑80 (MQ=255)                                           tatGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGACCGa                           <  2:115292/81‑1 (MQ=255)                                             tgtgTTAAGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGACCGaa                          <  1:115293/80‑1 (MQ=255)                                                   aaGCCTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGACCGAAGCTAtt                    <  1:115294/80‑1 (MQ=255)                                                     gCCTTCTTCGGAATCTTTTGTGTTTAGAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGACCGAAGCTATtgtg                 <  1:115295/81‑1 (MQ=255)                                                      ccTTCTTCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGACCGAAGCTATtgtg                 >  2:115314/1‑80 (MQ=255)                                                       cttcttCGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGACCGAAGCTATTGTGca               >  1:476238/1‑81 (MQ=255)                                                        ttcttcGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGACCGAAGCTATTGTGca               >  1:115333/1‑80 (MQ=255)                                                         tcttcGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGACCGAAGCTATTGTGcac              >  1:115319/1‑80 (MQ=255)                                                          cttcGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGACCGAAGCTATTGTGcac              <  1:115296/79‑1 (MQ=255)                                                            tcGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGACCGAAGCTATTGTGCACAACt          >  1:115337/1‑81 (MQ=255)                                                            tcGGAATCTTTTGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGACCGAAGCTATTGTGCACAACt          >  2:115324/1‑81 (MQ=255)                                                                      ttGTGTTTAAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGACCGAAGCTATTGTGCACAACTCCATCGcc  >  2:115323/1‑79 (MQ=255)                                                                              |                                                                       CTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGATTGTAAATGCGCATCAGTTTTTGACCGAAGCTATTGTGCACAACTCCATCGCC  >  NC_000913/582190‑582337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑