Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	585,573	A→G	S21P (TCT→CCT)	ompT ←	outer membrane protease VII (outer membrane protein 3b)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	585,573	0	A	G	100.0%	88.5 / NA	26	S21P (TCT→CCT)	ompT	outer membrane protease VII (outer membrane protein 3b)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/15);  total (11/15)

TTGTTTTTCCGCTCAGAGTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTT  >  NC_000913/585498‑585640                                                                            |                                                                    ttGTTTTTCCGCTCAGAGTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGGAGCa                                                                 >  1:115655/1‑80 (MQ=255)         ccGCTCAGAGTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCt                                                         >  2:115654/1‑80 (MQ=255)         ccGCTCAGAGTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCt                                                         <  1:115620/80‑1 (MQ=255)            cTCAGAGTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATc                                                     <  1:115621/81‑1 (MQ=255)            cTCAGAGTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATc                                                     <  1:115622/81‑1 (MQ=255)               agagTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCa                                                  <  2:115623/81‑1 (MQ=255)                gagTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCaa                                                 <  1:115624/81‑1 (MQ=255)                gagTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCaa                                                 >  2:115647/1‑81 (MQ=255)                   ttCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAg                                              <  1:115625/81‑1 (MQ=255)                      cAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAgggg                                           <  1:115626/81‑1 (MQ=255)                      cAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAgggg                                           >  2:115653/1‑81 (MQ=255)                           cTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAGGGGTTGt                                       >  2:115649/1‑80 (MQ=255)                                   cGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTAt                              <  1:115627/81‑1 (MQ=255)                                       tttATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTccc                          <  2:115628/81‑1 (MQ=255)                                         tATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTcc                           >  2:115659/1‑78 (MQ=255)                                         tATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCa                         <  2:115629/80‑1 (MQ=255)                                           tgttgtCAGGAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAg                        >  2:115660/1‑79 (MQ=255)                                            gttgtCAGAAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAg                     >  2:115663/1‑81 (MQ=255)                                                    gAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGccc             <  2:115630/81‑1 (MQ=255)                                                    gAGTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGccc             >  2:115657/1‑81 (MQ=255)                                                      gTAAACGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGc           <  1:115631/81‑1 (MQ=255)                                                         aaCGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCAt         <  2:115632/80‑1 (MQ=255)                                                         aaCGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCAt         >  2:115658/1‑80 (MQ=255)                                                          aCGATAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATaa       <  1:115633/81‑1 (MQ=255)                                                              tAAAGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGt   <  1:115634/81‑1 (MQ=255)                                                               aaaGTCTCGGTAGGAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATATAAGtt  >  2:115656/1‑81 (MQ=255)                                                                            |                                                                    TTGTTTTTCCGCTCAGAGTTCCAAGACTAATGTCCGCATTTATGTTGTCAGGAGTAAACGATAAAGTCTCGGTAGAAGCAAAAGAGCTGATCGCAATAGGGGTTGTCAGGACTATTCCCAGAAGTTTCGCCCGCATAAAAGTT  >  NC_000913/585498‑585640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑