Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	612,345	A→G	V50V (GTT→GTC)	fepA ←	ferric enterobactin outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	612,345	0	A	G	100.0%	103.9 / NA	30	V50V (GTT→GTC)	fepA	ferric enterobactin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/13);  total (17/13)

CATGGTACGGATGATCTTCGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGG  >  NC_000913/612273‑612422                                                                         |                                                                              cATGGTACGGATGATCTTCGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAgg                                                                       >  1:120525/1‑81 (MQ=255)    ggTACGGATGATCTTCGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGgcgc                                                                    >  1:120540/1‑81 (MQ=255)        cGGATGATCTTCGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTg                                                                 >  1:120535/1‑80 (MQ=255)                   cGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTc                                                          <  1:120498/76‑1 (MQ=255)                   cGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCt                                                         <  2:120499/77‑1 (MQ=255)                   cGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTc                                                     >  2:120532/1‑81 (MQ=255)                    gACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTcg                                                    >  2:120546/1‑81 (MQ=255)                    gACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTc                                                     <  1:120500/80‑1 (MQ=255)                     acacATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTcgg                                                   <  2:120501/81‑1 (MQ=255)                     acacATCGCGGGCAACCGGGCTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTcgg                                                   >  1:120539/1‑81 (MQ=255)                             cgGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGt                                            >  2:120541/1‑80 (MQ=255)                               ggCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAAc                                         <  1:120502/81‑1 (MQ=255)                                  aaCCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGa                                       >  1:120552/1‑80 (MQ=255)                                  aaCCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGAc                                      <  1:120503/81‑1 (MQ=255)                                    ccGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACaa                                    >  1:120533/1‑81 (MQ=255)                                       ggTTTTTGCGGATTTCATCTGCGGTGATTGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAg                                 >  2:120542/1‑81 (MQ=255)                                           tttGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTAt                              <  1:120504/80‑1 (MQ=255)                                           tttGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGCTCTGCTCGGCGGCGGTAACGACAATAGTATc                             >  2:120547/1‑81 (MQ=255)                                            ttGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCg                            >  1:120549/1‑81 (MQ=255)                                            ttGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCg                            >  1:120548/1‑81 (MQ=255)                                                  aTTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCAtgt                      <  2:120505/81‑1 (MQ=255)                                                   tttCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCAtgt                      >  2:120538/1‑80 (MQ=255)                                                    ttCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCAtgtg                     >  2:120545/1‑80 (MQ=255)                                                       aTCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGaaa                  <  2:120506/80‑1 (MQ=255)                                                       aTCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGaaa                  <  1:120507/80‑1 (MQ=255)                                                       aTCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGaaa                  >  1:120553/1‑80 (MQ=255)                                                             ggTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGt           <  1:120508/81‑1 (MQ=255)                                                                    gTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTAtcggtc    <  1:120509/81‑1 (MQ=255)                                                                     tCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTAtcggtcg   <  2:120510/81‑1 (MQ=255)                                                                      cGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTAtcggtcgg  >  2:120543/1‑81 (MQ=255)                                                                         |                                                                              CATGGTACGGATGATCTTCGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGG  >  NC_000913/612273‑612422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑