Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	633,421	T→C	P19P (CCA→CCG)	hcxA ←	hydroxycarboxylate dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	633,421	0	T	C	100.0%	85.1 / NA	25	P19P (CCA→CCG)	hcxA	hydroxycarboxylate dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/12);  total (13/12)

GCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGT  >  NC_000913/633344‑633496                                                                              |                                                                            gcgcGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCgg                                                                           <  2:124562/80‑1 (MQ=255)                  ccACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGc                                                         >  2:124611/1‑81 (MQ=255)                   cacaCCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGc                                                         <  1:124564/80‑1 (MQ=255)                   cacaCCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGc                                                         <  2:124563/80‑1 (MQ=255)                       ccGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGc                                                    >  2:792843/1‑81 (MQ=255)                          cgcgAGAAAGTTGTTCATCAGTGAAAAAATCGTTCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCcga                                                 >  2:124606/1‑81 (MQ=255)                            cgAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCcgacg                                               <  1:124565/81‑1 (MQ=255)                            cgAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCcgac                                                >  1:124607/1‑80 (MQ=255)                              agaAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCgacgac                                             <  2:124566/81‑1 (MQ=255)                                 aaGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCAc                                          >  1:124600/1‑81 (MQ=255)                                  aGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCAcg                                         >  2:124605/1‑81 (MQ=255)                                   gttgttCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCAcgc                                        <  2:124567/81‑1 (MQ=255)                                     tgttCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCAcgcg                                       >  1:124612/1‑80 (MQ=255)                                       ttCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGa                                     >  1:124602/1‑80 (MQ=255)                                       ttCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGAt                                    <  1:124568/81‑1 (MQ=255)                                          atcaGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAgg                                 <  2:124569/81‑1 (MQ=255)                                             aGTGAAAAAATCGTGCAGGTGATTGAAACTGCCCGGATGTGAAAAGAAGTTAGCCGGGCCGACGACCACGCGGATAGGAtt                              >  1:124599/1‑81 (MQ=255)                                                    aaaTCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGc                       <  1:124570/81‑1 (MQ=255)                                                      aTCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCAt                     >  1:124614/1‑81 (MQ=255)                                                         gTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCAtgtt                  >  2:124620/1‑81 (MQ=255)                                                                tGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATgttgtgttgt           <  1:124571/81‑1 (MQ=255)                                                                  aTTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTcc         >  1:124616/1‑81 (MQ=255)                                                                       aaCTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTAtt    >  1:124618/1‑81 (MQ=255)                                                                        aCTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTg   <  1:124572/81‑1 (MQ=255)                                                                         cTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGt  <  2:124573/81‑1 (MQ=255)                                                                              |                                                                            GCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGT  >  NC_000913/633344‑633496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑