Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	635,957	A→G	Y205H (TAC→CAC)	ybdN ←	putative PAPS reductase/DUF3440 domain‑containing protein YbdN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	635,957	0	A	G	100.0%	99.2 / NA	29	Y205H (TAC→CAC)	ybdN	putative PAPS reductase/DUF3440 domain‑containing protein YbdN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/22);  total (7/22)

TACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACGTTGTTTATTTAAACTGG  >  NC_000913/635881‑636034                                                                             |                                                                              taCAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATg                                                                           <  1:124987/81‑1 (MQ=255)   cAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGt                                                                          <  1:124988/80‑1 (MQ=255)     gTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGTAcc                                                                       <  2:124989/81‑1 (MQ=255)            gCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGTACCAGCTAt                                                                 >  2:125029/1‑80 (MQ=255)             cAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGTACCAGCTATg                                                                <  2:124990/80‑1 (MQ=255)              agagACGTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGGCGTAAATGGGGTGAATGTACCAGCTATGAc                                                              <  2:124991/81‑1 (MQ=255)               gagaCTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGTACCAGCTATGAcc                                                             <  1:124992/81‑1 (MQ=255)                   cTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGTACCAGCTATGACCGCCt                                                         <  1:124993/81‑1 (MQ=255)                        atgatTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGTACCAGCTATGACCGCCTGGTGc                                                    >  1:125024/1‑81 (MQ=255)                         tgatTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGTACCAGCTATGACCGCCTGGTGCg                                                   >  1:125015/1‑81 (MQ=255)                         tgatTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGTACCAGCTATGACCGCCTGGTGCg                                                   >  1:125028/1‑81 (MQ=255)                          gatTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGTACCAGCTATGACCGCCTGGTGCgg                                                  >  2:125021/1‑81 (MQ=255)                           atTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGTACCAGCTATGACCGCCTGGTGCGGc                                                 <  2:124994/81‑1 (MQ=255)                                cATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGTACCAGCTATGACCGCCTGGTGCGGCCGTa                                             <  1:124995/80‑1 (MQ=255)                                    ccAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCa                                        <  1:124996/81‑1 (MQ=255)                                     cAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAg                                       <  1:124997/81‑1 (MQ=255)                                       ggTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAggg                                     <  2:124999/81‑1 (MQ=255)                                       ggTACAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAggg                                     <  2:124998/81‑1 (MQ=255)                                         tCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTGAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGtt                                   <  1:125000/81‑1 (MQ=255)                                                     ccACTTTCCAGTCGTAAATGGGGTGAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCaa                       <  1:125001/81‑1 (MQ=255)                                                         tttCCAGTCGTAAATGGGGTGAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACg                   >  2:125035/1‑81 (MQ=255)                                                               gTCGTAAATGGGGTGAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACGTTGttt             <  2:125002/81‑1 (MQ=255)                                                                  gTAAATGGGGTGAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACGTTGtttatt          >  2:125026/1‑81 (MQ=255)                                                                   tAAATGGGGTGAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACGTTGtttattt         <  1:125003/81‑1 (MQ=255)                                                                     aaTGGGGTGAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACGTTGTTTATTTaa       <  1:125004/81‑1 (MQ=255)                                                                       tGGGGTGAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACGTTGTTTATTTAAAc     <  1:125005/81‑1 (MQ=255)                                                                         gggTGAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACGTTGTTTATTTAAACtg   <  2:125006/81‑1 (MQ=255)                                                                         gggTGAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACGTTGTTTATTTAAACt    <  2:125007/80‑1 (MQ=255)                                                                          ggTGAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACGTTGTTTATTTAAACtgg  <  1:125008/81‑1 (MQ=255)                                                                             |                                                                              TACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACGTTGTTTATTTAAACTGG  >  NC_000913/635881‑636034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑