Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	653,615	T→C	F461L (TTC→CTC)	dpiB →	sensory histidine kinase DpiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	653,615	0	T	C	100.0%	121.9 / NA	35	F461L (TTC→CTC)	dpiB	sensory histidine kinase DpiB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/19);  total (16/19)

TGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTATTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGTTCCAGAGTCTCTAC  >  NC_000913/653542‑653687                                                                          |                                                                         tgtgGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAg                                                                   >  2:128106/1‑81 (MQ=255)  gtgGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGc                                                                  >  2:128111/1‑81 (MQ=255)    gggCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGa                                                                >  1:128123/1‑81 (MQ=255)      gCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATg                                                              <  2:128069/81‑1 (MQ=255)      gCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATg                                                              <  2:128068/81‑1 (MQ=255)        aaTTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATg                                                              >  1:128104/1‑79 (MQ=255)            tACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGc                                                         <  2:128070/80‑1 (MQ=255)             aCTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCg                                                        <  1:128071/80‑1 (MQ=255)                  aTAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGatgatg                                                  >  2:128124/1‑81 (MQ=255)                        ccTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGa                                            >  1:128120/1‑81 (MQ=255)                            cGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGAtt                                          <  2:128073/79‑1 (MQ=255)                            cGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGAtt                                          <  1:128072/79‑1 (MQ=255)                            cGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTg                                         >  2:128110/1‑80 (MQ=255)                             gAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTg                                         >  2:128109/1‑79 (MQ=255)                                gccagccTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTc                                    >  2:128113/1‑81 (MQ=255)                                gccagccTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTc                                    <  2:128074/81‑1 (MQ=255)                                 ccagccTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCg                                   <  1:128075/81‑1 (MQ=255)                                  cagccTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGc                                  >  1:128126/1‑81 (MQ=255)                                  cagccTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGc                                  <  1:128076/81‑1 (MQ=255)                                      cTGGGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGAt                              <  2:128077/81‑1 (MQ=255)                                        gCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGAt                              >  1:128119/1‑79 (MQ=255)                                         cGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATc                             <  2:128078/79‑1 (MQ=255)                                         cGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATc                             <  1:128079/79‑1 (MQ=255)                                              cGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTg                      >  2:128121/1‑81 (MQ=255)                                               gATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTgc                     <  1:128080/81‑1 (MQ=255)                                                aTGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTgcg                    <  2:128081/81‑1 (MQ=255)                                                 tGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTgcgg                   <  2:128082/81‑1 (MQ=255)                                                    aGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGt                <  2:128083/81‑1 (MQ=255)                                                     ggAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGtt               <  2:128084/81‑1 (MQ=255)                                                      gAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGTTc              <  1:128085/81‑1 (MQ=255)                                                       aaaCAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGTTc              >  2:128112/1‑80 (MQ=255)                                                            aGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGTTCCAGAGt        >  2:128115/1‑81 (MQ=255)                                                                 gTTGAATTACTCCTCAGCGATGAAGGC‑‑‑GATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGTTCCAGAGTCTCTAc  >  1:128122/1‑79 (MQ=255)                                                                  ttGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGTTCCAGAGTCTCTAc  <  1:128086/81‑1 (MQ=255)                                                                     aaTTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGTTCCAGAGTCTCTAc  >  2:128117/1‑78 (MQ=255)                                                                          |                                                                         TGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTATTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGTTCCAGAGTCTCTAC  >  NC_000913/653542‑653687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑