Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	673,832	T→A	I318F (ATT→TTT)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	673,832	0	T	A	100.0%	93.6 / NA	27	I318F (ATT→TTT)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/15);  total (12/15)

CTGGTCGTGCCCCGGTACCGCCATAACTGCGCCCGTGCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCTTTTTTCTCCATCGTCGCCATTTCAGCTT  >  NC_000913/673755‑673909                                                                              |                                                                              cTGGTCGTGCCCCGGTACCGCCATAACTGCGCCCGTGCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAAAttc                                                                            <  2:131556/81‑1 (MQ=255) cTGGTCGTGCCCCGGTACCGCCATAACTGCGCCCGTGCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAAAttc                                                                            >  1:131594/1‑81 (MQ=255)         gCCCCGGTACCGCCATAACTGCGCCCGTGCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAAATTCTTCGCCCg                                                                    >  1:131599/1‑81 (MQ=255)           cccGGTACCGCCATAACTGCGCCCGTGCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAAATTCTTCGCCCGtt                                                                  <  2:131557/81‑1 (MQ=255)              ggTACCGCCATAACTGCGCCCGTGCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAAATTCTTCGCCCGTTAAt                                                               <  1:131558/81‑1 (MQ=255)                tACCGCCATAACTGCGCCCGTGCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAAATTCTTCGCCCGTTAATgg                                                             <  1:131559/81‑1 (MQ=255)                  ccGCCATAACTGCGCCCGTGCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAAATTCTTCGCCCGTTAATGGGt                                                           <  1:131560/81‑1 (MQ=255)                       aTAACTGCGCCCGTGCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAAATTCTTCGCCCGTTAATGGGTGAAcc                                                      <  1:131561/81‑1 (MQ=255)                          aCTGCGCCCGTGCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAAATTCTTCGCCCGTTAATGGGTGAACCGCt                                                   >  1:131597/1‑81 (MQ=255)                          aCTGCGCCCGTGCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAAATTCTTCGCCCGTTAATGGGTGAACCGCt                                                   <  1:131562/81‑1 (MQ=255)                              cgcCCGTGCCGTACTCCATCAATACGAAGTTTGCTTCCCAAACGGGAAATTCTTCGCCCGTTAATGGGTGAACCGCTTTa                                                >  1:131622/1‑80 (MQ=255)                              cgcACGTGCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAAATTCTTCGCCCGTTAATGGGTGAACCGCTTTaa                                               <  2:131563/81‑1 (MQ=255)                                 ccGTGCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAAATTCTTCGACCGTTAATGGGTGAACCGCTTTAAAGc                                            <  2:131564/81‑1 (MQ=255)                                   gTGCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAAATTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCa                                          <  2:131565/81‑1 (MQ=255)                                       cGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAAATTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTAt                                      >  2:131612/1‑81 (MQ=255)                                        gTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAAATTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATc                                     >  1:131595/1‑81 (MQ=255)                                        gTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAAATTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATc                                     <  1:131566/81‑1 (MQ=255)                                                  aaTACGAAGTTTGCTGCCCAAACGGGAAATTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCttt                            <  1:131567/80‑1 (MQ=255)                                                        aaGTTTGCTGCCCAAACGGGAAATTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCTTTTTtctc                      >  1:131614/1‑80 (MQ=255)                                                         aGTTTGCTGCCCAAACGGGAAATTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCTTTTTTCTCCa                    >  2:131600/1‑81 (MQ=255)                                                          gTTTGCTGCCCAAACGGGAAATTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCTTTTTTCTCCAt                   >  2:131610/1‑81 (MQ=255)                                                            ttGCTGCCCAAACGGGAAATTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCTTTTTTCTCCAtcg                 >  1:131617/1‑81 (MQ=255)                                                               ctgcCCAAACGGGAAATTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCTTTTTTCTCCAtcgtc               <  1:131568/80‑1 (MQ=255)                                                                tgcCCAAACGGGAAATTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCTTTTTTCTCCATCGTCGc             >  2:131606/1‑81 (MQ=255)                                                                     aaaCGGGAAATTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCTTTTTTCTCCATCGTCGCCAttt        <  1:131569/81‑1 (MQ=255)                                                                      aaCGGGAAATTCTTCGCCCGTTAATGAGTGAACCGCTTTAAAGCCAGTATCGACGCCTTTTTTCTCCATCGTCGCCATTTc       <  2:131570/81‑1 (MQ=255)                                                                           gAAATTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCTTTTTTCTCCATCGTCGCCATTTCAGCtt  >  1:131598/1‑81 (MQ=255)                                                                              |                                                                              CTGGTCGTGCCCCGGTACCGCCATAACTGCGCCCGTGCCGTACTCCATCAATACGAAGTTTGCTGCCCAAACGGGAATTTCTTCGCCCGTTAATGGGTGAACCGCTTTAAAGCCAGTATCGACGCCTTTTTTCTCCATCGTCGCCATTTCAGCTT  >  NC_000913/673755‑673909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑