Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	697,144	(T)8→7	intergenic (‑11/+369)	metT ← / ← asnB	tRNA‑Met/asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	697,137	0	T	.	100.0%	96.2 / NA	24	intergenic (‑4/+376)	metT/asnB	tRNA‑Met/asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (7/17);  total (7/17)

TGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCA  >  NC_000913/697057‑697213                                                                                 |                                                                             tggtggCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCt                                                                              <  1:149871/81‑1 (MQ=33)      gCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCttttt                                                                          <  1:149872/80‑1 (MQ=40)        tACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCtttttttc                                                                       >  1:149913/1‑80 (MQ=255)           gacgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCtttttttcgcg                                                                    <  1:149873/81‑5 (MQ=255)           gacgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCtttttttcgc                                                                     >  1:149912/1‑77 (MQ=255)             cgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGa                                                                  >  1:149919/1‑80 (MQ=255)                    agAACCTGTGACCCCATCATTATGAGTGATGGGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTa                                                           <  2:149874/79‑1 (MQ=17)                     gAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATc                                                         >  2:149909/1‑81 (MQ=255)                            tgACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTg                                                  <  2:149875/81‑1 (MQ=255)                              aCCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGc                                                 <  2:149876/80‑1 (MQ=255)                               ccccATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCgg                                               >  1:149911/1‑81 (MQ=255)                                 ccATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCgggg                                             <  2:149877/81‑1 (MQ=255)                                 ccATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCgggg                                             <  1:149878/81‑1 (MQ=255)                                    tcatTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGgcgc                                          <  1:149879/81‑1 (MQ=255)                                           gAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGc                                   >  1:149915/1‑81 (MQ=255)                                            aGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCg                                  <  1:149880/81‑1 (MQ=255)                                                aTGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGtata                              <  2:149881/81‑1 (MQ=255)                                                   tgCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATaga                            <  2:149882/80‑1 (MQ=255)                                                   tgCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATaga                            <  1:149883/80‑1 (MQ=255)                                                    gCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGc                          >  1:149918/1‑81 (MQ=255)                                                        tAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCt                        <  1:149884/79‑1 (MQ=255)                                                         aaCCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTg                      >  2:149920/1‑80 (MQ=255)                                                          aCCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCtt                       <  1:149885/78‑1 (MQ=255)                                                          aCCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGc                     <  2:149886/80‑1 (MQ=255)                                                              aCTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGt                <  1:149888/81‑1 (MQ=255)                                                              aCTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGt                <  2:149887/81‑1 (MQ=255)                                                                 gAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTCTGCGTATAGAGCCTTGCAGCGTCaa             >  2:149922/1‑81 (MQ=255)                                                                   gCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAAcc           <  1:149889/81‑1 (MQ=255)                                                                            ccATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCa  <  1:149890/81‑1 (MQ=255)                                                                                 |                                                                             TGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTCTTTTTCA  >  NC_000913/697057‑697213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑