Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	723,868	(G)5→4	coding (547/2685 nt)	kdpD ←	sensory histidine kinase KdpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	723,864	0	G	.	100.0%	115.2 / NA	28	coding (551/2685 nt)	kdpD	sensory histidine kinase KdpD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (18/10);  total (18/10)

GGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCCGGGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAAT  >  NC_000913/723784‑723937                                                                                 |                                                                          ggAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCCg                                                                           >  1:154707/1‑81 (MQ=255)       aaTGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCCTTCAGCCGCTGGCGCAGATCGTCCggggc                                                                       >  2:154714/1‑78 (MQ=255)          gTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTc                                                                 >  2:154717/1‑81 (MQ=255)             cAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTtcac                                                              >  1:154709/1‑81 (MQ=255)             cAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTccac                                                              >  2:154719/1‑81 (MQ=255)                  gcgcgcTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGca                                                         >  1:154713/1‑81 (MQ=255)                    gcgcTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGcac                                                        >  1:154715/1‑80 (MQ=255)                       cTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCAcg                                                    <  1:154666/81‑1 (MQ=255)                        tCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCAcgt                                                   >  1:154727/1‑81 (MQ=255)                         ccGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCAcgtc                                                  >  2:154710/1‑81 (MQ=255)                         ccGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCAcgtc                                                  >  2:154721/1‑81 (MQ=255)                         ccGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCAcgtc                                                  >  1:154708/1‑81 (MQ=255)                           gCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCAcgtcgt                                                <  1:154667/81‑1 (MQ=255)                                cccGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTcggc                                            <  2:154669/80‑1 (MQ=255)                                cccGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTcggc                                            <  2:154668/80‑1 (MQ=255)                                cccGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTcggc                                            <  1:154670/80‑1 (MQ=255)                                 ccGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTcggcgg                                          <  1:154671/81‑1 (MQ=255)                                        tataGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGaa                                   <  1:154672/81‑1 (MQ=255)                                           aGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGaaaa                                 >  1:154711/1‑80 (MQ=255)                                                  gCCTTCTTTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCg                         >  2:154716/1‑81 (MQ=255)                                                      tcttTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCa                     >  2:154702/1‑81 (MQ=255)                                                      tcttTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCa                     >  1:154722/1‑81 (MQ=255)                                                       cttTCAGCCGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCAc                    >  1:154737/1‑81 (MQ=255)                                                               cGCTGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTccc            >  2:154725/1‑81 (MQ=255)                                                                  tGGCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTa         >  1:154729/1‑81 (MQ=255)                                                                   ggCGCAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTAc        >  1:154718/1‑81 (MQ=255)                                                                    gcgcAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTAcc       <  1:154674/81‑1 (MQ=255)                                                                    gcgcAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTAcc       <  1:154673/81‑1 (MQ=255)                                                                      gcAGATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCt      >  1:154728/1‑80 (MQ=255)                                                                         gATCGTCC‑GGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAAt  <  1:154675/81‑1 (MQ=255)                                                                                 |                                                                          GGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTTTCAGCCGCTGGCGCAGATCGTCCGGGGGCAAGTCCACCAGCACCACGTCGTCGGCGGCATCGAAAAAAGGATCGGGCACGGTTTCCCGTACCTGAAT  >  NC_000913/723784‑723937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑