Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	728,334	Δ1 bp	coding (399/1674 nt)	kdpA ←	K(+) transporting P‑type ATPase subunit KdpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	728,333	0	T	.	100.0%	120.0 / NA	29	coding (400/1674 nt)	kdpA	K(+) transporting P‑type ATPase subunit KdpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (10/19);  total (10/19)

ATGCTCTGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTG  >  NC_000913/728254‑728403                                                                                |                                                                       aTGCTCTGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGta                                                                       <  2:155668/81‑2 (MQ=255)   gCTCTGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGtaag                                                                     >  2:155711/1‑78 (MQ=255)   gCTCTGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGtaag                                                                     >  1:155721/1‑78 (MQ=255)          cGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCa                                                             <  1:155670/81‑1 (MQ=255)           gggTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCAt                                                            >  2:155716/1‑81 (MQ=255)               aaaCGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTgg                                                        >  1:155706/1‑81 (MQ=255)                  cGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCt                                                      >  2:155707/1‑80 (MQ=255)                  cGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTg                                                     <  1:155671/81‑1 (MQ=255)                     aCGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGaaa                                                  <  2:155672/81‑1 (MQ=255)                       ggATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGaaa                                                  >  2:155718/1‑79 (MQ=255)                        gATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAg                                               <  1:155673/81‑1 (MQ=255)                         aTGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGc                                              <  2:155674/81‑1 (MQ=255)                             gggCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCaa                                          >  1:155712/1‑81 (MQ=255)                              ggaaaaaaTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAAc                                         <  1:155675/78‑1 (MQ=255)                               gCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACg                                        <  1:155676/81‑1 (MQ=255)                                cAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGt                                       <  2:155677/81‑1 (MQ=255)                                 aaaaaTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGTg                                      <  2:155678/81‑1 (MQ=255)                                     aTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGttt                                  >  2:155728/1‑81 (MQ=255)                                      tCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGttt                                  >  2:155726/1‑80 (MQ=255)                                      tCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTc                                 <  2:155680/81‑1 (MQ=255)                                      tCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTc                                 <  1:155679/81‑1 (MQ=255)                                          cGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCg                             >  1:155724/1‑81 (MQ=255)                                              aTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCtat                         <  1:155681/81‑1 (MQ=255)                                              aTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCta                          >  2:155714/1‑80 (MQ=255)                                                   gCTGGCGGCAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGAt                    >  1:155727/1‑81 (MQ=255)                                                       gcggcAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTg                  <  1:155683/79‑1 (MQ=255)                                                       gcggcAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGcc                <  2:155682/81‑1 (MQ=255)                                                           cAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGtt            <  1:155685/81‑1 (MQ=255)                                                           cAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGtt            <  2:155686/81‑1 (MQ=255)                                                           cAGAAAGAAAGTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGtt            <  1:155684/81‑1 (MQ=255)                                                              aaagaaagTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGt         <  2:155687/81‑1 (MQ=255)                                                                     gTTTTGCACCG‑TAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTg  <  2:155688/81‑1 (MQ=255)                                                                                |                                                                       ATGCTCTGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTG  >  NC_000913/728254‑728403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑