Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	742,314	(C)5→4	coding (243/1482 nt)	dtpD ←	dipeptide:H(+) symporter DtpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	742,310	0	C	.	100.0%	120.0 / NA	29	coding (247/1482 nt)	dtpD	dipeptide:H(+) symporter DtpD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (9/20);  total (9/20)

ATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACC  >  NC_000913/742231‑742386                                                                                |                                                                             aTCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGcc                                                                             >  2:158120/1‑81 (MQ=255)  tCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGccc                                                                            >  1:158128/1‑81 (MQ=255)   cGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGccc                                                                            >  1:158134/1‑80 (MQ=255)    gCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGcccca                                                                          <  2:158078/81‑2 (MQ=255)    gCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGcccc                                                                           <  1:158079/80‑1 (MQ=255)      cAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACTTGACCGATCGCCATCAACAACGccccag                                                                         >  1:158129/1‑78 (MQ=255)        gggACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCAt                                                                     <  1:158080/81‑1 (MQ=255)         ggACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATc                                                                    <  2:158081/81‑1 (MQ=255)            cAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCAcc                                                                 >  1:158124/1‑81 (MQ=255)                  gagGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCAt                                                            <  1:158083/80‑1 (MQ=255)                  gagGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCAt                                                            <  2:158082/80‑1 (MQ=255)                    ggAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCg                                                         <  2:158084/81‑1 (MQ=255)                           cGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGcc                                                   >  2:158137/1‑80 (MQ=255)                             gatggatCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCgag                                                <  2:158086/81‑1 (MQ=255)                             gatggatCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCgag                                                <  2:158085/81‑1 (MQ=255)                             gatggatCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACAGCCATGCGATTGcc                                                   >  2:158126/1‑78 (MQ=255)                                 gatCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAAc                                             <  2:158087/80‑1 (MQ=255)                                       aCTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATcc                                      <  2:158089/81‑1 (MQ=255)                                       aCTGGCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATcc                                      <  2:158088/81‑1 (MQ=255)                                           gCACCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGcc                                   <  2:158090/80‑1 (MQ=255)                                             aCCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCaaa                                <  1:158091/81‑1 (MQ=255)                                             aCCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCaa                                 >  1:158139/1‑80 (MQ=255)                                             aCCCAGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCaa                                 >  1:158138/1‑80 (MQ=255)                                                 aGCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGc                            <  1:158092/81‑1 (MQ=255)                                                  gCACCACATGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGcc                           <  1:158093/81‑1 (MQ=255)                                                      cacaTGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCg                       <  2:158094/81‑1 (MQ=255)                                                       acaTGACCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCAcc                        >  2:158143/1‑79 (MQ=255)                                                            aCCGATCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGAtt                 >  1:158125/1‑81 (MQ=255)                                                                 tCGCCATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGt            <  2:158095/81‑1 (MQ=255)                                                                    ccATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGa          >  2:158144/1‑80 (MQ=255)                                                                    ccATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGAc         <  1:158096/81‑1 (MQ=255)                                                                    ccATCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGAc         <  1:158097/81‑1 (MQ=255)                                                                      aTCAACAACG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACAt       <  2:158098/81‑1 (MQ=255)                                                                          acaacG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATacac   >  2:158123/1‑81 (MQ=255)                                                                           caacG‑CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACAcc  <  1:158099/81‑1 (MQ=255)                                                                                |                                                                             ATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACC  >  NC_000913/742231‑742386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑