Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	842,838	(T)5→4	coding (507/927 nt)	rlmF →	23S rRNA m(6)A1618 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	842,834	0	T	.	100.0%	93.8 / NA	23	coding (503/927 nt)	rlmF	23S rRNA m(6)A1618 methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (8/15);  total (8/15)

AGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGATTTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGACCTTGTGTAACCCGCCATTCCACGATTCCGCCGC  >  NC_000913/842755‑842909                                                                                |                                                                            aGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGAtt                                                                            >  2:177407/1‑81 (MQ=255)   tGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGAtttt                                                                          <  1:177365/81‑1 (MQ=255)    gcgcAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGAtttt                                                                          >  2:177404/1‑80 (MQ=255)    gcgcAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGAtttt                                                                          >  1:177408/1‑80 (MQ=255)        aGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACgg                                                                    >  1:177402/1‑81 (MQ=255)        aGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACgg                                                                    >  1:177403/1‑81 (MQ=255)                 tCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATcc                                                            <  2:177366/80‑1 (MQ=255)                   aattCTAATCCGGGGCTTAACCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATa                                                         <  2:177367/79‑1 (MQ=255)                          aTCCGGGGCTTAACCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAAc                                                    <  1:177368/79‑1 (MQ=255)                           tCCGGGGCTTAACCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAg                                                 <  2:177369/81‑1 (MQ=255)                             cGGGGCTTAACCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAGc                                                <  1:177370/80‑1 (MQ=255)                              ggggCTTAACCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAGCaa                                              >  2:177400/1‑81 (MQ=255)                                  cTTAACCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAGCAATacg                                          <  1:177371/81‑1 (MQ=255)                                     aaCCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAGCAATacgac                                        <  2:177372/80‑1 (MQ=255)                                      aCCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAGCAATACGAcgc                                      >  1:177417/1‑81 (MQ=255)                                        cgcgCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGa                                    >  1:177412/1‑81 (MQ=255)                                            ccATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGACCtt                                <  2:177373/81‑1 (MQ=255)                                               ttCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGACCTtgtg                             <  2:177374/81‑1 (MQ=255)                                                 cGTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGACCTTgtgt                            >  1:177413/1‑80 (MQ=255)                                                  gTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGACCTTGTGTaa                          <  1:177376/81‑1 (MQ=255)                                                  gTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGACCTTGTGTaa                          <  1:177378/81‑1 (MQ=255)                                                  gTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGACCTTGTGTaa                          <  2:177375/81‑1 (MQ=255)                                                  gTCTGCGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGACCTTGTGTa                           <  1:177377/80‑1 (MQ=255)                                                       cGTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGACCTTGTGTAACCCGc                     <  2:177379/81‑1 (MQ=255)                                                        gTCGGCAAAAAGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGACCTTGTGTAACCCGc                     >  1:177416/1‑80 (MQ=255)                                                                aaaGAGAGTGGGGCGA‑TTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGACCTTGTGTAACCCGCCATTCCACg            <  2:177380/81‑1 (MQ=255)                                                                          gggCGA‑TTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGACCTTGTGTAACCCGCCATTCCACGATTCcgccgc  >  1:177411/1‑81 (MQ=255)                                                                                |                                                                            AGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCTGCGTCGGCAAAAAGAGAGTGGGGCGATTTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGACCTTGTGTAACCCGCCATTCCACGATTCCGCCGC  >  NC_000913/842755‑842909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑