Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	78,635	(G)5→4	coding (1015/1179 nt)	setA →	sugar exporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	78,631	0	G	.	100.0%	66.5 / NA	17	coding (1011/1179 nt)	setA	sugar exporter SetA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (6/11);  total (6/11)

TTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTG  >  NC_000913/78558‑78707                                                                          |                                                                             ttAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCggggcag                                                                        <  2:19239/80‑4 (MQ=255)   aaCGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGC‑GGGGCAGCTa                                                                    >  1:19277/1‑81 (MQ=255)    aCGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGC‑GGGGCAGCTa                                                                    <  1:19240/80‑1 (MQ=255)       cTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGC‑GGGGCAGCTaccac                                                                <  1:19241/81‑1 (MQ=255)          tATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGC‑GGGGCAGCTACCACCtt                                                             <  1:19242/81‑1 (MQ=255)               aTCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGC‑GGGGCAGCTACCACCTTAttt                                                         <  2:19243/80‑1 (MQ=255)                tCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGC‑GGGGCAGCTACCACCTTATTTAc                                                       <  1:19244/81‑1 (MQ=255)                 cGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGC‑GGGGCAGCTACCACCTTATTTAc                                                       >  2:19274/1‑80 (MQ=255)                  ggCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGC‑GGGGCAGATACCACCTTATTTACTa                                                     <  2:19245/81‑1 (MQ=255)                   gCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGC‑GGGGCAGCTACCACCTTATTTACTaa                                                    >  1:19263/1‑81 (MQ=255)                    cATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGC‑GGGGCAGCTACCACCTTATTTACTaa                                                    >  2:19275/1‑80 (MQ=255)                              ggTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGC‑GGGGCAGCTACCACCTTATTTACTAACAGTATTTCTa                                         <  2:19246/81‑1 (MQ=255)                               gTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGC‑GGGGCAGCTACCACCTTATTTACTAACAGTATTTCTAc                                        >  1:19280/1‑81 (MQ=255)                                tATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGC‑GGGGCAGCTACCACCTTATTTACTAACAGTATTTCTAcc                                       <  2:19247/81‑1 (MQ=255)                                   tGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGC‑GGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCggg                                    >  2:19281/1‑81 (MQ=255)                                             tGGTTTCAGGATTTAATGCCTGGAAGAGC‑GGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTctgg                          <  1:19248/81‑1 (MQ=255)                                               gTTTCAGGATTTAATGCCTGGAAGAGC‑GGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTctggct                        <  2:19249/81‑1 (MQ=255)                                                                     agagC‑GGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTg  <  2:19250/81‑1 (MQ=255)                                                                          |                                                                             TTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTG  >  NC_000913/78558‑78707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑