Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	911,171	Δ1 bp	intergenic (‑122/+11)	poxB ← / ← hcr	pyruvate oxidase/NADH oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	911,169	0	G	.	100.0%	93.8 / NA	23	intergenic (‑120/+13)	poxB/hcr	pyruvate oxidase/NADH oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (15/8);  total (15/8)

CATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATT  >  NC_000913/911093‑911245                                                                             |                                                                             cATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCggca                                                                           >  2:189632/1‑78 (MQ=255) cATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCggca                                                                           >  2:189612/1‑78 (MQ=255)  aTCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCggcata                                                                         <  2:189585/81‑5 (MQ=255)       aCGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAgg                                                                     <  2:189586/79‑1 (MQ=255)         ggAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGAt                                                                 >  2:189624/1‑81 (MQ=255)             ggggAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATg                                                             >  2:529885/1‑81 (MQ=255)             ggggAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATg                                                             <  1:189587/81‑1 (MQ=255)                gAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGc                                                            >  2:189629/1‑79 (MQ=255)                     gATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAAcc                                                     <  2:189588/81‑1 (MQ=255)                     gATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAAcc                                                     <  2:189589/81‑1 (MQ=255)                        gggAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCaaa                                                  >  2:189621/1‑81 (MQ=255)                         ggAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAAt                                                 >  1:189634/1‑81 (MQ=255)                               aaaGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCt                                           >  2:189630/1‑81 (MQ=255)                                aaGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTg                                          >  2:189631/1‑81 (MQ=255)                                  ggaggCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCg                                        >  1:189627/1‑81 (MQ=255)                                       cACTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATgg                                   >  2:189620/1‑81 (MQ=255)                                        aCTAACGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATgg                                   >  1:189625/1‑80 (MQ=255)                                            aCGGTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAgg                               <  1:189590/80‑1 (MQ=255)                                              ggTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAg                             >  2:189628/1‑80 (MQ=255)                                              ggTTAAATAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGc                            <  1:189591/81‑1 (MQ=255)                                                    aTAGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGc                        >  2:189642/1‑79 (MQ=255)                                                      aGCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAg                     >  1:189623/1‑80 (MQ=255)                                                       gCCCGATGAAAGGAATATCATC‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTa                   >  2:189618/1‑81 (MQ=255)                                                                       atcatc‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGAt   >  2:189633/1‑81 (MQ=255)                                                                        tcatc‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGAtt  <  1:189592/81‑1 (MQ=255)                                                                        tcatc‑GGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGAtt  <  2:189593/81‑1 (MQ=255)                                                                             |                                                                             CATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATT  >  NC_000913/911093‑911245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑