Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	966,010	Δ1 bp	coding (1691/2265 nt)	ycaI →	conserved inner membrane protein YcaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	966,007	0	G	.	100.0%	85.0 / NA	21	coding (1688/2265 nt)	ycaI	conserved inner membrane protein YcaI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (13/8);  total (13/8)

ACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATGGATCAGAAGTCCGTTAAACTGGGAACATCATCAG  >  NC_000913/965927‑966083                                                                                 |                                                                             acaacTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCg                                                                              <  2:199215/81‑1 (MQ=255)  caacTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGTATCACCgg                                                                             >  2:199257/1‑81 (MQ=255)   aacTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCggg                                                                            <  1:199216/81‑1 (MQ=255)   aacTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCggg                                                                            <  2:199217/81‑1 (MQ=255)    acTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCggga                                                                           <  1:199218/81‑2 (MQ=255)    acTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCgg                                                                             >  1:199264/1‑79 (MQ=255)    acTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCgg                                                                             >  2:199253/1‑79 (MQ=255)              cccTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACC‑GGGAGGGCTGGAt                                                                 <  2:199219/80‑1 (MQ=255)                 tGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACC‑GGGAGGGCTGGATTCaa                                                             >  1:199249/1‑81 (MQ=255)                       cACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACC‑GGGAGGGCTGGATTCAATATTg                                                        >  2:199254/1‑80 (MQ=255)                       cACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACC‑GGGAGGGCTGGATTCAATATTg                                                        >  2:199251/1‑80 (MQ=255)                              aTAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACC‑GGGAGGGCTGGATTCAATATTGCATATATg                                                >  1:285615/1‑81 (MQ=255)                               tAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACC‑GGGAGGGCTGGATTCAATATTGCATATATgg                                               <  2:199220/81‑1 (MQ=255)                                   cTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACC‑GGGAGGGCTGGATTCAATATTGCATATATGGCCGa                                           >  2:199256/1‑81 (MQ=255)                                      gAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACC‑GGGAGGGCTGGATTCAATATTGCATATATGGCCGATg                                         <  1:199221/80‑1 (MQ=255)                                        aCCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACC‑GGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTa                                      <  1:199222/81‑1 (MQ=255)                                           ggAAGGCGTTATTCTGAGCCATGAACATCTGGATCACC‑GGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATg                                    >  2:199260/1‑80 (MQ=255)                                              aGGCGTTATTCTGAGCCATGAACATCTGGATCACC‑GGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATGGATc                                <  2:199223/81‑1 (MQ=255)                                                         tGAGCCATGAACATCTGGATCACC‑GGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATGGATCAGAAGTCCGt                      >  1:470039/1‑80 (MQ=255)                                                           aGCCATGAACATCTGGATCACC‑GGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATGGATCAGAAGTCCGtt                     <  2:199224/79‑1 (MQ=255)                                                           aGCCATGAACATCTGGATCACC‑GGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATGGATCAGAAGTCCGtt                     <  1:199225/79‑1 (MQ=255)                                                            gCCATGAACATCTGGATCACC‑GGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATGGATCAGAAGTCCGTTaaa                  <  1:199226/81‑1 (MQ=255)                                                                 gAACATCTGGATCACC‑GGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATGGATCAGAAGTCCGTTAAACTgg              >  2:199268/1‑80 (MQ=255)                                                                   aCATCTGGATCACC‑GGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATGGATCAGAAGTCCGTTAAACTGGGaa           >  1:199248/1‑81 (MQ=255)                                                                       cTGGATCACC‑GGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATGGATCAGAAGTCCGTTAAACTGGGAAcat        >  1:199261/1‑80 (MQ=255)                                                                        tGGATCACC‑GGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATGGATCAGAAGTCCGTTAAACTGGGAAcatca      >  1:199275/1‑81 (MQ=255)                                                                        tGGATCACC‑GGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATGGATCAGAAGTCCGTTAAACTGGGAAcatca      >  2:199265/1‑81 (MQ=255)                                                                            tCACC‑GGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATGGATCAGAAGTCCGTTAAACTGGGAACATCATCAg  >  2:199263/1‑81 (MQ=255)                                                                                 |                                                                             ACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTGGATCACCGGGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATGGATCAGAAGTCCGTTAAACTGGGAACATCATCAG  >  NC_000913/965927‑966083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑