Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,146,603	(T)7→6	noncoding (15/169 nt)	sraB →	small RNA SraB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,146,597	0	T	.	100.0%	85.1 / NA	21	noncoding (9/169 nt)	sraB	small RNA SraB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (9/12);  total (9/12)

CATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTT  >  NC_000913/1146519‑1146670                                                                               |                                                                          caTTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATAttt                                                                         <  1:229627/81‑1 (MQ=255)   ttCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATAttttt                                                                       <  1:229628/81‑1 (MQ=255)           agaTTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAg                                                              <  1:229629/81‑1 (MQ=255)             aTTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGa                                                             >  2:229669/1‑80 (MQ=255)                    gTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGt                                                     <  2:229630/81‑1 (MQ=255)                         tacAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATtg                                                 <  2:229631/78‑1 (MQ=255)                         tCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATtga                                                >  1:229661/1‑81 (MQ=255)                           cAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATtgatg                                              <  2:229632/81‑1 (MQ=255)                             gaagaGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTgatgat                                            <  2:229633/81‑1 (MQ=255)                                    gcgACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCAtt                                     >  1:229667/1‑81 (MQ=255)                                         gCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTaaa                                >  1:229652/1‑81 (MQ=255)                                         gCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTaaa                                <  1:229634/81‑1 (MQ=255)                                          ccATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAAc                               <  1:229635/81‑1 (MQ=255)                                               gCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCTTCAGATATAGCGTATTGATGATAGCCATTTTAAACTATg                           <  1:229637/80‑1 (MQ=255)                                               gCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATgc                          >  1:229663/1‑81 (MQ=255)                                               gCGAGGTGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATg                           <  1:229636/80‑1 (MQ=255)                                                     tGGAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCg                    >  2:229674/1‑81 (MQ=255)                                                      ggAGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGt                   >  1:229682/1‑81 (MQ=255)                                                        aGGCTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGttt                 <  2:229638/81‑1 (MQ=255)                                                           cTAAAATAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGc              <  2:229639/81‑1 (MQ=255)                                                              aaaTAAGTTTAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCa             <  1:229640/79‑1 (MQ=255)                                                                     tttAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGAtgt    >  1:229665/1‑81 (MQ=255)                                                                       tAGGCATA‑TTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGAtgtct  >  2:229659/1‑79 (MQ=255)                                                                               |                                                                          CATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTT  >  NC_000913/1146519‑1146670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑