| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,182,642 | Δ1 bp | coding (188/1047 nt) | potD ← | spermidine preferential ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,182,640 | 0 | T | . | 93.8% | 53.4 / ‑1.7 | 16 | coding (190/1047 nt) | potD | spermidine preferential ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (8/7); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
ATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATAGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGG > NC_000913/1182561‑1182715 | aTTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATAGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGtt > 1:235684/1‑81 (MQ=255) tATCAACGTAATAGGTTGAAGGAACCACCAGATCATAGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGttcgtt > 2:235690/1‑77 (MQ=255) tCAACGTAATAGGTTGAAGGAACCACCAGATCATAGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGg‑ttcgttc < 1:235660/80‑1 (MQ=255) aGGTTGAAGGAACCACCAGATCATAGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGG‑TTCGTTCGACTCGTAAGt > 2:235674/1‑81 (MQ=255) gTTGAAGGAACCACCAGATCATAGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGG‑TTCGTTCGACTCGTAAGTc > 2:235689/1‑80 (MQ=255) aaGGAACCACCAGATCATAGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGG‑TTCGTTCGACTCGTAAGTCGaa > 2:235696/1‑79 (MQ=255) aaGGAACCACCAGATCATAGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGG‑TTCGTTCGACTCGTAAGTCGAAt < 1:235661/80‑1 (MQ=255) aaGGAACCACCAGATCATAGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGG‑TTCGTTCGACTCGTAAGTCGAATa < 1:235662/81‑1 (MQ=255) cATAGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGG‑TTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATAcc > 2:235691/1‑81 (MQ=255) cACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGG‑TTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTc < 1:235663/81‑1 (MQ=255) cGTCTTTGTATGTTTTCAGCTTGGCGTACATGG‑TTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCttt > 1:235688/1‑81 (MQ=255) gTCTTTGTATGTTTTCAGCTTGGCGTACATGG‑TTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTg < 2:235664/81‑1 (MQ=255) tGTATGTTTTCAGCTTGGCGTACATGG‑TTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGa < 2:235665/80‑1 (MQ=255) ttttCAGCTTGGCGTACATGG‑TTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGtt > 1:235693/1‑80 (MQ=255) tCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTc > 1:235704/1‑79 (MQ=255) aGCTTGGCGTACATGG‑TTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGc < 2:235666/80‑1 (MQ=255) tACATGG‑TTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTggcg < 1:235667/81‑1 (MQ=255) aCATGG‑TTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTggcgg > 2:235694/1‑81 (MQ=255) aCATGG‑TTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTggcgg > 2:235695/1‑81 (MQ=255) | ATTTTATCAACGTAATAGGTTGAAGGAACCACCAGATCATAGGCACCGTCTTTGTATGTTTTCAGCTTGGCGTACATGGTTTCGTTCGACTCGTAAGTCGAATAGATAACCTTAATACCGGTTTCTTTGGTGAACTGTTCAAGCAGTCCTGGCGG > NC_000913/1182561‑1182715 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |