| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,185,245 | (T)6→5 | coding (350/1137 nt) | potA ← | spermidine preferential ABC transporter ATP binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,185,240 | 0 | T | . | 96.2% | 99.3 / ‑1.3 | 26 | coding (355/1137 nt) | potA | spermidine preferential ABC transporter ATP binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (9/16); minor base G (1/0); total (10/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
GTTTGCGTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAA > NC_000913/1185162‑1185314 | gTTTGCGTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGttt < 2:236141/81‑1 (MQ=255) ttGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATg > 2:236193/1‑81 (MQ=255) gAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATgc < 1:236143/80‑1 (MQ=255) aGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAAccc < 2:236144/81‑1 (MQ=255) cAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAATGcc < 2:236145/81‑1 (MQ=255) cTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCcaca < 2:236146/81‑1 (MQ=255) cACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACAttt < 2:236147/81‑1 (MQ=255) aCCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACAtttt > 1:236192/1‑81 (MQ=255) aTCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCg > 2:236205/1‑80 (MQ=255) cGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAaca < 2:236148/81‑1 (MQ=255) gCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAacac > 2:236187/1‑81 (MQ=255) gCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAacac < 2:236149/81‑1 (MQ=255) cAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACg < 1:236150/81‑1 (MQ=255) gggCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGt < 1:236151/81‑1 (MQ=255) cTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCAt < 1:236152/81‑1 (MQ=255) ggggCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAg < 2:236153/81‑1 (MQ=255) gggCGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGt > 1:236191/1‑81 (MQ=255) cGTAATTTCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCg > 2:236182/1‑81 (MQ=255) tCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCtt < 1:236155/80‑1 (MQ=255) tCAGCAGCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCtt < 1:236154/80‑1 (MQ=255) cagcagCGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTg > 2:236197/1‑81 (MQ=255) agcagcGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTgg < 1:236156/81‑1 (MQ=255) agcagcGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTg < 1:236157/80‑1 (MQ=255) cagcGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGaa < 1:460615/81‑1 (MQ=255) agcGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGaa > 1:236213/1‑80 (MQ=255) agcGGGGG‑TTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGaa > 2:236208/1‑80 (MQ=255) gcggggGTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGaaaa > 1:236186/3‑81 (MQ=255) | GTTTGCGTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAA > NC_000913/1185162‑1185314 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |