Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,251,091	Δ1 bp	coding (26/1920 nt)	dhaR →	DNA‑binding transcriptional dual regulator DhaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,251,090	0	G	.	100.0%	102.6 / NA	25	coding (25/1920 nt)	dhaR	DNA‑binding transcriptional dual regulator DhaR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (13/12);  total (13/12)

TATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCTGATGAAACGGGAGACATGGAAC  >  NC_000913/1251011‑1251164                                                                                |                                                                           tATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATg                                                                            <  1:245472/80‑1 (MQ=255)       tACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGc                                                                    <  2:245473/81‑1 (MQ=255)        aCTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCa                                                                   >  2:666858/1‑81 (MQ=255)            aaaaTTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCatat                                                                <  1:245474/80‑1 (MQ=255)            aaaaTTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCATAtc                                                               >  2:245512/1‑81 (MQ=255)             aaaTTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCATAtct                                                              >  1:245503/1‑81 (MQ=255)                      gCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAAtt                                                     <  1:245475/81‑1 (MQ=255)                               ccTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAAcctcc                                            >  1:245504/1‑81 (MQ=255)                                cTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAAcctcct                                           <  2:245476/81‑1 (MQ=255)                                         tAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGAt                                  <  1:245477/81‑1 (MQ=255)                                          aaGGGATGCGGGATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATg                                 >  2:245498/1‑81 (MQ=255)                                               aTGCGGGATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAAt                             >  1:245506/1‑80 (MQ=255)                                               aTGCGGGATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATa                            <  2:245478/81‑1 (MQ=255)                                                  cGGGATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGc                         >  1:245499/1‑81 (MQ=255)                                                   gggATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCt                        >  1:245507/1‑81 (MQ=255)                                                    ggATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCtg                       <  2:245480/81‑1 (MQ=255)                                                    ggATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCtg                       <  1:245479/81‑1 (MQ=255)                                                    ggATATGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCt                        >  1:245514/1‑80 (MQ=255)                                                        atGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCtgatg                    >  2:245500/1‑80 (MQ=255)                                                        atGAGTGGCGCTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCtgatg                    >  2:245508/1‑80 (MQ=255)                                                               gcgcTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCTGATGAAACGGga            <  1:245481/81‑1 (MQ=255)                                                                 gcTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCTGATGAAACGGgaga          >  1:245517/1‑81 (MQ=255)                                                                  cTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCTGATGAAACGGgaga          >  2:245511/1‑80 (MQ=255)                                                                  cTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCTGATGAAACGGGAGAc         <  2:245482/81‑1 (MQ=255)                                                                  cTTTTAACAACGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCTGATGAAACGGGAGAc         <  2:245483/81‑1 (MQ=255)                                                                         caacGAT‑GTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCTGATGAAACGGGAGACATGGAAc  <  1:245484/81‑1 (MQ=255)                                                                                |                                                                           TATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCTGATGAAACGGGAGACATGGAAC  >  NC_000913/1251011‑1251164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑