Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,314,871	(T)6→5	coding (354/507 nt)	yciE ←	DUF892 domain‑containing protein YciE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,314,866	0	T	.	100.0%	41.1 / NA	11	coding (359/507 nt)	yciE	DUF892 domain‑containing protein YciE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (4/7);  total (4/7)

GAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCT  >  NC_000913/1314789‑1314930                                                                              |                                                                 gAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCAtttt                                                               >  2:255110/1‑81 (MQ=255)  aaTCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCAttttt                                                              <  1:255088/81‑1 (MQ=255)   aTCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCAtttttg                                                             >  1:255102/1‑80 (MQ=255)     cAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCAtttttgct                                                           >  2:255100/1‑78 (MQ=255)      aGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCAtttttgctg                                                          >  1:255109/1‑77 (MQ=255)                     tGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAg                                           <  2:255089/80‑1 (MQ=255)                               cATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGc                                >  2:255108/1‑81 (MQ=255)                                     aaaTCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGAttt                           >  1:255107/1‑80 (MQ=255)                                     aaaTCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTc                          <  1:255090/81‑1 (MQ=255)                                              cGATGGTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGc                   <  1:255091/79‑1 (MQ=255)                                               gATGGTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCg                <  1:255092/81‑1 (MQ=255)                                                  ggTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCg                >  2:255104/1‑78 (MQ=255)                                                   gTTGGAATTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAg             <  2:255093/80‑1 (MQ=255)                                                    ttGGAACTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGAc           <  1:255094/81‑1 (MQ=255)                                                         aTTGAAGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATc      <  2:255095/81‑1 (MQ=255)                                                              aGCTGTATCACCGGCA‑TTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCt  >  2:255105/1‑80 (MQ=255)                                                                              |                                                                 GAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTTCGATGGTTGGAATTGAAGCTGTATCACCGGCATTTTTTGCTGCTGCTAATAGTGAGGTGTAACAGGCGATTTCAAATTGCTCGAAGACATATCCGCT  >  NC_000913/1314789‑1314930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑