Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,365,665	Δ1 bp	coding (116/1266 nt)	puuE →	4‑aminobutyrate aminotransferase PuuE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,365,663	0	G	.	100.0%	89.5 / NA	22	coding (114/1266 nt)	puuE	4‑aminobutyrate aminotransferase PuuE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (16/6);  total (16/6)

TGCCACTCCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGCCACCCTGATCTGGTCGCG  >  NC_000913/1365585‑1365738                                                                               |                                                                            tGCCACTCCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAgg                                                                            >  2:263009/1‑80 (MQ=255)   ccACTCCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAggc                                                                           <  1:262958/79‑2 (MQ=255)   ccACTCCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAggc                                                                           <  2:262959/79‑2 (MQ=255)   ccACTCCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAgg                                                                            <  2:262957/78‑1 (MQ=255)     aCTCCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAggcaac                                                                        >  1:262987/1‑76 (MQ=255)                      cGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTcgcc                                                     >  1:262999/1‑81 (MQ=255)                       gTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTcgccg                                                    >  1:262995/1‑81 (MQ=255)                                  cttcttCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCg                                         >  2:262992/1‑81 (MQ=255)                                   ttcttcGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCgg                                        >  1:263013/1‑81 (MQ=255)                                        cGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCt                                    >  2:263019/1‑80 (MQ=255)                                        cGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCt                                    >  2:262994/1‑80 (MQ=255)                                         gCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTg                                   <  1:262960/80‑1 (MQ=255)                                             aGTCGGCTGAAAACGCCACGCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATAc                              >  1:263000/1‑81 (MQ=255)                                              gTCGGCTGAAAACGCCACGCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATAcc                             >  1:263002/1‑81 (MQ=255)                                                      aaaaCGCCACGCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACAt                       <  1:262961/79‑1 (MQ=255)                                                       aaaCGCCACGCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGc                    >  2:263014/1‑81 (MQ=255)                                                        aaCGCCACGCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGcc                   <  2:262962/81‑1 (MQ=255)                                                        aaCGCCACGCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGcc                   <  2:262963/81‑1 (MQ=255)                                                        aaCGCCACGCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGc                    >  1:263006/1‑80 (MQ=255)                                                         aCGCCACGCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGCCa                  >  1:263022/1‑81 (MQ=255)                                                            ccACGCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGCCAccc               >  1:262998/1‑81 (MQ=255)                                                                gCTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGCCACCCTGAt           >  1:263005/1‑81 (MQ=255)                                                                 cTGAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGCCACCCTGATc          >  2:263010/1‑81 (MQ=255)                                                                   gAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGCCACCCTGATCTg        >  2:262989/1‑81 (MQ=255)                                                                   gAAGGATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGCCACCCTGATCTg        >  2:263004/1‑81 (MQ=255)                                                                       gATGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGCCACCCTGATCTGGTcg    <  1:262964/81‑1 (MQ=255)                                                                         tGTTGA‑GGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGCCACCCTGATCTGGTcgcg  <  1:262965/81‑1 (MQ=255)                                                                               |                                                                            TGCCACTCCGCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCAGTCGGCTGAAAACGCCACGCTGAAGGATGTTGAGGGCAACGAGTACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATACCGGACATCGCCACCCTGATCTGGTCGCG  >  NC_000913/1365585‑1365738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑