Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	152,807	(T)5→4	coding (6/570 nt)	yadM ←	putative fimbrial protein YadM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	152,803	0	T	.	100.0%	97.7 / NA	24	coding (10/570 nt)	yadM	putative fimbrial protein YadM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (12/12);  total (12/12)

AAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTATGTGGCGTTGTTTTTATCATTTGACGTCCCTTGTAGTTACTGAATCTGACACCGAATTCCATTCAGAATAATAGATTGTGCTATTTT  >  NC_000913/152723‑152879                                                                                 |                                                                             aaaCTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTATGTGGCgttgt                                                                              >  1:36721/1‑81 (MQ=255)           ttAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCAttt                                                                   >  2:36691/1‑81 (MQ=255)           ttAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCAttt                                                                   <  2:36685/81‑1 (MQ=255)             aaTATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCATTTg                                                                  <  1:36686/80‑1 (MQ=255)              atatCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCATTTGAc                                                                >  1:36723/1‑81 (MQ=255)               tatCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCATTTGACg                                                               >  2:36720/1‑81 (MQ=255)                 tCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCATTTGACGTc                                                             <  1:36687/81‑1 (MQ=255)                  ccGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCATTTGACGTcc                                                            >  1:36725/1‑81 (MQ=255)                     ttGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCATTTGACGTCCCtt                                                         >  1:36731/1‑81 (MQ=255)                            tACTGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCATTTGACGTCCCTTGTAGTTa                                                  <  2:36688/81‑1 (MQ=255)                              cTGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCATTTGACGTCCCTTGTAGTTACt                                                >  2:36722/1‑81 (MQ=255)                               tGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCATTTGACGTCCCTTGTAGTTACt                                                >  1:36729/1‑80 (MQ=255)                                  gggTGATAATAATATTCCCATCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCATTTGACGTCCCTTGTAGTTACTGAAt                                            <  2:36689/81‑1 (MQ=255)                                   ggTGATAATAATATTCCCATCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCATTTGACGTCCCTTGTAGTTACTGAATc                                           >  1:36719/1‑81 (MQ=255)                                         aataataTTCCCATCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCATTTGACGTCCCTTGTAGTTACTGAATCTGacac                                     >  1:36726/1‑81 (MQ=255)                                          ataataTTCCCATCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCATTTGACGTCCCTTGTAGTTACTGAATCTGACAcc                                    <  2:36690/81‑1 (MQ=255)                                             atatTCCCATCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCATTTGACGTCCCTTGTAGTTACTGAATCTGACACCGa                                  <  1:36691/80‑1 (MQ=255)                                                     aTCAGTATCACTATTTTATGTGGCGTTG‑TTTTATCATTTGACGTCCCTTGTAGTTACTGAATCTGACACCGAATTCCATTc                         <  2:36692/81‑1 (MQ=255)                                                          tATCACTATTTTATGTGGCGTTG‑TTTTATCATTTGACGTCCCTTGTAGTTACTGAATCTGACACCGAATTCCATTCAGaat                    <  2:575541/81‑1 (MQ=255)                                                                  ttttATGTGGCGTTG‑TTTTATCATTTGACGTCCCTTGTAGTTACTGAATCTGACACCGAATTCCATTCAGAATAATAGAtt            >  1:36733/1‑81 (MQ=255)                                                                   tttATGTTGCGTTG‑TTTTATCATTTGACGTCCCTTGTAGTTACTGAATCTGACACCGAATTCCATTCAGAATAATAGATtg           >  1:36741/1‑81 (MQ=255)                                                                   tttATGTGGCGTTG‑TTTTATCATTTGACGTCCCTTGTAGTTACTGAATCTGACACCGAATTCCATTCAGAATAATAGATtg           >  2:36724/1‑81 (MQ=255)                                                                    ttATGTGGCGTTG‑TTTTATCATTTGACGTCCCTTGTAGTTACTGAATCTGACACCGAATTCCATTCAGAATAATAGATtg           <  1:36693/80‑1 (MQ=255)                                                                           gCGTTG‑TTTTATCATTTGACGTCCCTTGTAGTTACTGAATCTGACACCGAATTCCATTCAGAATAATAGATTGTGCTAtt    <  2:36694/80‑1 (MQ=255)                                                                            cGTTG‑TTTTATCATTTGACGTCCCTTGTAGTTACTGAATCTGACACCGAATTCCATTCAGAATAATAGATTGTGCTAtttt  <  2:36695/81‑1 (MQ=255)                                                                                 |                                                                             AAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTATGTGGCGTTGTTTTTATCATTTGACGTCCCTTGTAGTTACTGAATCTGACACCGAATTCCATTCAGAATAATAGATTGTGCTATTTT  >  NC_000913/152723‑152879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑