Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,077	Δ1 bp	coding (131/906 nt)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,076	0	G	.	100.0%	89.3 / NA	22	coding (130/906 nt)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (10/12);  total (10/12)

CCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGC  >  NC_000913/1381996‑1382152                                                                                 |                                                                             ccTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATg                                                                              <  1:265603/81‑1 (MQ=255)               gAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTAT‑GCGAAGATatggatg                                                               >  1:265638/1‑81 (MQ=255)                 ggaaaggaaCGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTAT‑GCGAAGATATGGATggg                                                             >  2:265645/1‑81 (MQ=255)                                   cTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTc                                           <  1:265604/81‑1 (MQ=255)                                          atCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTAtttt                                     >  1:265644/1‑80 (MQ=255)                                           tCGGCGCGATGTACTAAATAGAAAACGTCGAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTa                                    <  1:265605/80‑1 (MQ=255)                                           tCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTaa                                   <  1:265606/81‑1 (MQ=255)                                             ggCGCGATGTACGAAATAGAAAACGTCGAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATg                                 >  1:265639/1‑81 (MQ=255)                                                gcgATGTACGAAATAGAAAACGTCGAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATgccg                              >  1:265635/1‑81 (MQ=255)                                                 cgATGTACGAAATAGAAAACGTCGAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATgccgc                             <  2:265607/81‑1 (MQ=255)                                                  gATGTACGAAATAGAAAACGTCGAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATgccgc                             >  1:265641/1‑80 (MQ=255)                                                    tGTACGAAATAGAAAACGTCGAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCaa                          <  2:265608/81‑1 (MQ=255)                                                    tGTACGAAATAGAAAACGTCGAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCaa                          <  1:265609/81‑1 (MQ=255)                                                        cGAAATAGAAAACGTCGAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGACCCTTCAGTCTATTTTAATGCCGCCAACgg                       <  2:265610/80‑1 (MQ=255)                                                         gAAATAGAAAACGTCGAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGc                     <  2:265611/81‑1 (MQ=255)                                                           aaTAGAAAACGTCGAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCg                   <  1:265612/81‑1 (MQ=255)                                                               gAAAACGTCGAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTgg                <  2:265613/80‑1 (MQ=255)                                                                 aaaCGTCGAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGgaga             <  1:265614/81‑1 (MQ=255)                                                                       cGAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGct       >  2:265657/1‑81 (MQ=255)                                                                        gAGGGTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGctc      <  1:265615/81‑1 (MQ=255)                                                                          gggTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTg    >  2:265634/1‑81 (MQ=255)                                                                           ggTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTgg   >  1:265637/1‑81 (MQ=255)                                                                            gTTAT‑GCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGc  >  1:265643/1‑81 (MQ=255)                                                                                 |                                                                             CCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCGAAGATATGGATGGGCTGGCGGAGCCTTCAGTCTATTTTAATGCCGCCAACGGGCCGTGGAGAATTGCTCTGGC  >  NC_000913/1381996‑1382152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑