Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,476,628	(A)6→5	coding (1485/2307 nt)	ydbD →	DUF2773 domain‑containing protein YdbD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,476,623	0	A	.	100.0%	36.8 / NA	10	coding (1480/2307 nt)	ydbD	DUF2773 domain‑containing protein YdbD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (5/5);  total (5/5)

GAATGACGATGATCTAATACGTGTGTTATTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAGAAAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGTAATTCACTCTGACGCTGCTTTACAACGGT  >  NC_000913/1476544‑1476692                                                                                |                                                                      gaATGACGATGATCTAATACGTGTGTTATTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGgaga                                                                       >  1:279317/1‑80 (MQ=255)                 aTACGTGTGTTATTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACt                                                     >  2:279323/1‑81 (MQ=255)                            aTTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCgg                                          >  2:279324/1‑81 (MQ=255)                             tttttAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCgg                                          <  2:279306/80‑1 (MQ=255)                              ttttAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGa                                        >  2:279322/1‑81 (MQ=255)                                         ccAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGTa                              <  2:279307/80‑1 (MQ=255)                                            gATAACTTACAGTTTAATTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATTCAGGTAATTc                          <  1:279308/81‑1 (MQ=255)                                                            aTTTTGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGTAATTCACTCTGACGCTGCtt           <  1:279309/80‑1 (MQ=255)                                                                tGTTAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGTAATTCACTCTGACGCTGCTTTACaa      >  1:279335/1‑81 (MQ=255)                                                                  ttAAAAGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGTAATTCACTCTGACGCTGCTTTACAAc     <  1:279310/80‑1 (MQ=255)                                                                    aaaaGGATGGAG‑AAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGTAATTCACTCTGACGCTGCTTTACAACGGt  >  1:279328/1‑81 (MQ=255)                                                                                |                                                                      GAATGACGATGATCTAATACGTGTGTTATTTTTAGCATTGCCAGATAACTTACAGTTTAATTTTGTTAAAAGGATGGAGAAAAAATCCCCGGCCTACTTTTGCTGTCGGGATATGCAGGTAATTCACTCTGACGCTGCTTTACAACGGT  >  NC_000913/1476544‑1476692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑