Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,481,929	(T)5→4	coding (1021/1293 nt)	ynbD →	putative phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,481,925	0	T	.	95.7%	85.2 / ‑1.2	23	coding (1017/1293 nt)	ynbD	putative phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base . (12/10);  minor base G (1/0);  total (13/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGG  >  NC_000913/1481846‑1481995                                                                                |                                                                       gACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTAtt                                                                       >  1:279937/1‑81 (MQ=255)      atTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTAttttgtg                                                                  >  1:279953/1‑78 (MQ=255)        tCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTA‑TTTTGTGTa                                                               >  1:279939/1‑81 (MQ=255)           ggCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTA‑TTTTGTGTAcc                                                             >  2:279935/1‑80 (MQ=255)            gCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTA‑TTTTGTGTACCGa                                                           >  1:279936/1‑81 (MQ=255)             cacaGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTA‑TTTTGTGTACCGAt                                                          <  1:279915/81‑1 (MQ=255)              acaGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCT‑GTTTTGTGTACCGATg                                                         >  1:279948/1‑81 (MQ=255)                  aaTGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTA‑TTTTGTGTACCGATGCTg                                                      >  2:279952/1‑80 (MQ=255)                        gTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTA‑TTTTGTGTACCGATGCTGGATCtgg                                               <  1:279916/81‑1 (MQ=255)                              gACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTA‑TTTTGTGTACCGATGCTGGATCTGGTGGTTc                                         <  2:279917/81‑1 (MQ=255)                              gACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTA‑TTTTGTGTACCGATGCTGGATCTGGTGGTTc                                         >  2:279949/1‑81 (MQ=255)                               aCGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTA‑TTTTGTGTACCGATGCTGGATCTGGTGGTTcc                                        <  1:279918/81‑1 (MQ=255)                                cGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTA‑TTTTGTGTACCGATGCTGGATCTGGTGGTTCCg                                       >  1:279961/1‑81 (MQ=255)                                    aCCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTA‑TTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGaa                                    <  1:279919/80‑1 (MQ=255)                                           aaTTCCCTCGCGGACGAGCCACAAAAGATCAACTCTA‑TTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAggggg                             <  2:279920/80‑1 (MQ=255)                                                     cgGACGAGCCACAAAAGATCGACTCTA‑TTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAg                  >  2:279950/1‑81 (MQ=255)                                                        aCGAGCCACAAAAGATCGACTCTA‑TTTTGTGTACCGATGCTGGACCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGcc               >  2:279963/1‑81 (MQ=255)                                                         cGAGCCACAAAAGATCGACTCTA‑TTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCg              >  1:279955/1‑81 (MQ=255)                                                          gAGCCACAAAAGATCGACTCTA‑TTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGt             <  2:279921/81‑1 (MQ=255)                                                          gAGCCACAAAAGATCGACTCTA‑TTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGt             >  1:279945/1‑81 (MQ=255)                                                            gCCACAAAAGATCGACTCTA‑TTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTgg           >  2:279960/1‑81 (MQ=255)                                                               acaAAAGATCGACTCTA‑TTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGa        <  2:693123/81‑1 (MQ=255)                                                                caAAAGATCGACTCTA‑TTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGAt       <  2:279922/81‑1 (MQ=255)                                                                  aaaGATCGACTCTA‑TTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGc     >  1:279954/1‑81 (MQ=255)                                                                     gATCGACTCTA‑TTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTgg  <  1:279923/81‑1 (MQ=255)                                                                                |                                                                       GACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGCTCCGACAGGCCGTGGCGATGCTGG  >  NC_000913/1481846‑1481995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑