Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,526,837	Δ1 bp	coding (591/618 nt)	yncG →	putative glutathione S‑transferase YncG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,526,836	0	A	.	100.0%	32.2 / NA	9	coding (590/618 nt)	yncG	putative glutathione S‑transferase YncG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (5/4);  total (5/4)

GGATAATGCCACGAATATCAGTGCGATTGCTGATGCTGTATGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTAT  >  NC_000913/1526774‑1526909                                                               |                                                                          ggATAATGCCACGAATATCAGTGCGATTGCTGATGCTGTATGTCAGTTACCGAAATTACAAG‑AGTATTAAAAAGGAATGaa                                                        <  1:286604/81‑1 (MQ=255)                tatCAGTGCGATTGCTGATGCTGTATGTCAGTTACCGAAATTACAAG‑AGTATTAAAAAGGAATGAAATTATTTGAGTGGGa                                         <  1:286605/81‑1 (MQ=255)                    aGTGCGATTGCTGATGCTGTATGTCAGTTACCGAAATTACAAG‑AGTATTAAAAAGGAATGAAATTATTTGAGTGGGATAcc                                     <  2:286606/81‑1 (MQ=255)                              cTGATGCTGTATGTCAGTTACCGAAATTACAAG‑AGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTAt                           >  2:286619/1‑81 (MQ=255)                                             aGTTACCGAAATTACAAG‑AGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTc             <  2:286607/80‑1 (MQ=255)                                               ttACCGAAATTACAAG‑AGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAg           >  1:286614/1‑80 (MQ=255)                                                 aCCGAAATTACAAG‑AGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTaa        >  1:286613/1‑81 (MQ=255)                                                    gAAATTACAAG‑AGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACtt     >  1:286617/1‑81 (MQ=255)                                                       aTTACAAG‑AGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTAt  >  2:286611/1‑81 (MQ=255)                                                               |                                                                          GGATAATGCCACGAATATCAGTGCGATTGCTGATGCTGTATGTCAGTTACCGAAATTACAAGAAGTATTAAAAAGGAATGAAATTATTTGAGTGGGATACCAGAAGATTATGTTATTTATTATTCAGTAACTTTAT  >  NC_000913/1526774‑1526909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑