Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,582,643	(A)6→5	intergenic (‑119/+283)	ydeN ← / ← ydeO	putative sulfatase/DNA‑binding transcriptional dual regulator YdeO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,582,638	0	A	.	100.0%	41.1 / NA	11	intergenic (‑114/+288)	ydeN/ydeO	putative sulfatase/DNA‑binding transcriptional dual regulator YdeO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (6/5);  total (6/5)

ACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTT  >  NC_000913/1582567‑1582711                                                                        |                                                                          aCGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTc                                                                 >  2:294315/1‑81 (MQ=255)    aaGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCAt                                                              <  1:294307/81‑1 (MQ=255)           aTAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGt                                                       <  1:294308/81‑1 (MQ=255)               gTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGAc                                                   <  2:294309/81‑1 (MQ=255)                tGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACa                                                  >  2:294316/1‑81 (MQ=255)                                tAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTAt                                  >  1:294314/1‑81 (MQ=255)                                      caTAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGt                             >  2:294317/1‑80 (MQ=255)                                       aTAAAGAGATAGCAGATTTAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTaa                           <  1:294310/81‑1 (MQ=255)                                                        ttAGCTAAAAAAAGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAaac           >  2:294318/1‑80 (MQ=255)                                                                aaaaaGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAAttt  <  1:294311/81‑1 (MQ=255)                                                                aaaaaGGG‑AAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAAtt   >  2:294321/1‑80 (MQ=255)                                                                        |                                                                          ACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTT  >  NC_000913/1582567‑1582711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑