| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,601,831 | (A)7→6 | coding (342/1536 nt) | lsrA → | Autoinducer‑2 ABC transporter ATP binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,601,825 | 0 | A | . | 96.0% | 93.0 / ‑1.9 | 25 | coding (336/1536 nt) | lsrA | Autoinducer‑2 ABC transporter ATP binding subunit |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base . (11/13); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
GGTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGG > NC_000913/1601748‑1601901 | ggTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCaa < 2:296729/79‑1 (MQ=255) tttATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCaaaaaac < 2:296730/80‑2 (MQ=255) ctcGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGctc > 1:296770/1‑81 (MQ=255) gTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGctc < 1:296731/77‑1 (MQ=255) gTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTcc < 1:296732/80‑1 (MQ=255) gTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTcc < 2:296733/80‑1 (MQ=255) aCCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATg < 2:296734/81‑1 (MQ=255) cGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGaaaaatga < 1:296735/81‑5 (MQ=255) cGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATg > 1:296769/1‑79 (MQ=255) gctgctTTTCCCAAGCCTGTCGATAAAAGAAGACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATgaag < 2:296736/81‑1 (MQ=255) tgctTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATgaagaa > 2:296765/1‑81 (MQ=255) tttCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTg > 1:296752/1‑81 (MQ=255) tCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGc < 1:296737/80‑1 (MQ=255) cAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTggc > 2:296767/1‑81 (MQ=255) aaGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTggcg < 2:296738/81‑1 (MQ=255) aGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTggcgg > 1:296761/1‑81 (MQ=255) tCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTgg < 1:296740/81‑1 (MQ=255) tCGACAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCt < 2:296739/79‑1 (MQ=255) tAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTg < 2:296741/81‑1 (MQ=255) aaaGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGc < 1:296742/80‑1 (MQ=255) aaGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGc > 1:296776/1‑79 (MQ=255) aCATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGa > 1:296785/1‑81 (MQ=255) cTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTg < 2:296743/80‑1 (MQ=255) tGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCa > 1:296779/1‑81 (MQ=255) gTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCa > 1:296768/1‑80 (MQ=255) gggCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTc > 2:296789/1‑81 (MQ=255) cTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTgg > 2:296775/1‑81 (MQ=255) | GGTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGG > NC_000913/1601748‑1601901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |