Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,739,579	(T)7→6	coding (220/933 nt)	ydhB ←	putative LysR family DNA‑binding transcriptional regulator YdhB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,739,573	0	T	.	100.0%	80.7 / NA	20	coding (226/933 nt)	ydhB	putative LysR family DNA‑binding transcriptional regulator YdhB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (11/9);  total (11/9)

ATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCG  >  NC_000913/1739497‑1739645                                                                             |                                                                         atatTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAttttt                                                                      <  1:315969/81‑1 (MQ=255)         cACTGCGATAGCTAACTGACCGCGCCAACCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCA‑TTTTTTGacaa                                                               >  1:316014/1‑79 (MQ=255)                   gCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCctt                                                   <  2:315970/81‑1 (MQ=255)                     tAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCcttct                                                 >  1:316016/1‑81 (MQ=255)                      aaCTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCcttctt                                                >  2:316020/1‑81 (MQ=255)                        cTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCttt                                               >  2:315978/1‑80 (MQ=255)                        cTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCTTTg                                              <  1:315971/81‑1 (MQ=255)                           aCCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCTTTgag                                            <  2:315972/80‑1 (MQ=255)                              gcgcCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCTTTGAGAAAc                                        >  2:316021/1‑81 (MQ=255)                                 ccAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCAc                                     >  1:911723/1‑81 (MQ=255)                                   aGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGc                                   >  1:316009/1‑81 (MQ=255)                                      cGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTcc                                <  2:315973/81‑1 (MQ=255)                                      cGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTc                                 >  1:316030/1‑80 (MQ=255)                                         tCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCcag                              >  2:316012/1‑80 (MQ=255)                                                  gctgACATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAg                    >  1:316011/1‑81 (MQ=255)                                                   ctgACATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGt                       <  1:315974/77‑1 (MQ=255)                                                      aCATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTcc                <  1:315975/81‑1 (MQ=255)                                                           gCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACAt            <  1:315976/80‑1 (MQ=255)                                                            cTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATc           <  2:315977/80‑1 (MQ=255)                                                               gCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCAc         <  1:315978/79‑1 (MQ=255)                                                                    tGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCcg  >  2:315979/1‑81 (MQ=255)                                                                             |                                                                         ATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCG  >  NC_000913/1739497‑1739645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑