Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,772,241	(A)7→6	coding (1171/1215 nt)	ydiM →	putative exporter YdiM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,772,235	0	A	.	100.0%	23.2 / NA	7	coding (1165/1215 nt)	ydiM	putative exporter YdiM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (4/3);  total (4/3)

TGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCA  >  NC_000913/1772156‑1772306                                                                                |                                                                        tGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCa                                                                         >  1:320711/1‑80 (MQ=255)  gCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCaa                                                                        >  2:320726/1‑80 (MQ=255)                gTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGC‑AAAAAACGCGGCATCAc                                                        >  2:320722/1‑81 (MQ=255)                    gATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGC‑AAAAAACGCGGCATCACTCGc                                                    >  2:320725/1‑81 (MQ=255)                                       tCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGC‑AAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTcc                                 >  2:320728/1‑81 (MQ=255)                                                  cTGGCACTGTTTATCGGCTTACGCAGCCGC‑AAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTaa                       <  2:320708/80‑1 (MQ=255)                                                       aCTGTTTATCGGCTTACGCAGCCGC‑AAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAat                 <  2:320709/81‑1 (MQ=255)                                                                 ggCTTACGCAGCCGC‑AAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCtttt         >  2:320727/1‑79 (MQ=255)                                                                       cGCAGCCGC‑AAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCa  <  2:320710/80‑1 (MQ=255)                                                                                |                                                                        TGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCA  >  NC_000913/1772156‑1772306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑