Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,790,524	Δ1 bp	coding (721/1437 nt)	ydiU ←	UPF0061 family protein YdiU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,790,521	0	C	.	100.0%	63.6 / NA	16	coding (724/1437 nt)	ydiU	UPF0061 family protein YdiU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (8/8);  total (8/8)

GTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTA  >  NC_000913/1790447‑1790588                                                                           |                                                                    gTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCAcccatg                                                                <  2:323411/80‑4 (MQ=255)     tcatcaAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCaa                                                          <  2:323412/81‑1 (MQ=255)       atcaAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCaaa                                                         <  2:323413/80‑1 (MQ=255)        tcaAGAGAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGc                                                       <  1:323415/81‑1 (MQ=255)        tcaAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGc                                                       <  2:323414/81‑1 (MQ=255)                 ccAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCt                                              <  2:323416/81‑1 (MQ=255)                   aaaCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGc                                            >  2:323435/1‑81 (MQ=255)                     aCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCa                                          >  1:323438/1‑81 (MQ=255)                       ggCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCAtt                                        >  1:323441/1‑81 (MQ=255)                           cGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGc                                    <  1:323417/81‑1 (MQ=255)                            gTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCa                                   <  2:323418/81‑1 (MQ=255)                              aaTCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAAt                                 >  1:323449/1‑81 (MQ=255)                              aaTCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAAt                                 >  2:323452/1‑81 (MQ=255)                                      gTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAacgacg                         >  1:323443/1‑81 (MQ=255)                                                     gACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGacaa          >  1:323446/1‑81 (MQ=255)                                                        aTGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACAt       <  2:323420/81‑1 (MQ=255)                                                              tCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTa  >  2:323495/1‑80 (MQ=255)                                                                           |                                                                    GTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTA  >  NC_000913/1790447‑1790588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑