Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	215,731	(T)5→4	coding (463/711 nt)	nlpE →	lipoprotein NlpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	215,727	0	T	.	100.0%	111.3 / NA	27	coding (459/711 nt)	nlpE	lipoprotein NlpE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (11/16);  total (11/16)

GAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACG  >  NC_000913/215650‑215797                                                                              |                                                                       gAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTAttt                                                                      <  2:51335/80‑1 (MQ=255)  aaTCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTAtttt                                                                     <  1:51336/80‑1 (MQ=255)     cGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTAttttatat                                                                 >  1:51378/1‑77 (MQ=255)          ttCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTg                                                           <  1:51337/81‑1 (MQ=255)           tCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGa                                                          >  1:51370/1‑81 (MQ=255)            cAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGa                                                          <  1:51338/80‑1 (MQ=255)             aaCTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGAt                                                         <  1:51339/80‑1 (MQ=255)              aCTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATgc                                                       <  2:51340/81‑1 (MQ=255)               cTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATgcg                                                      <  1:51341/81‑1 (MQ=255)                  taCGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATgcggc                                                    <  1:51342/80‑1 (MQ=255)                     gCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATGCGGCGAcc                                                <  2:51343/81‑1 (MQ=255)                        ggAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATGCGGCGACCtt                                              >  1:51373/1‑80 (MQ=255)                           aGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACt                                          >  2:51387/1‑81 (MQ=255)                                   aaTCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACTGATTgcgc                                  <  2:51344/81‑1 (MQ=255)                                    aTCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACTGATTGcgcg                                 <  2:51345/81‑1 (MQ=255)                                     tCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGa                                >  1:51371/1‑81 (MQ=255)                                       cAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGAcc                              >  2:51374/1‑81 (MQ=255)                                       cAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGAc                               <  2:51346/80‑1 (MQ=255)                                          tttACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCgg                            >  1:51389/1‑80 (MQ=255)                                          tttACCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGa                           <  2:51347/81‑1 (MQ=255)                                             aCCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATTGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGaaa                         <  2:51348/80‑1 (MQ=255)                                             aCCTATGACGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGaaaa                        >  2:51372/1‑81 (MQ=255)                                                    aCGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTc                  <  2:51349/80‑1 (MQ=255)                                                     cGCCGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCAt                <  1:51350/81‑1 (MQ=255)                                                        cGATGACCCTGCGGGGCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATgg              >  2:51384/1‑80 (MQ=255)                                                             aCCCTGCGGGGCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCg         >  1:51391/1‑80 (MQ=255)                                                             aCCCTGCGGGGCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCg         <  1:51351/80‑1 (MQ=255)                                                                cTGCGGAGCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATa     <  2:51352/81‑1 (MQ=255)                                                                    ggggCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACg  >  2:51381/1‑80 (MQ=255)                                                                      ggCATGTA‑TTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACg  >  2:51390/1‑78 (MQ=255)                                                                              |                                                                       GAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATGGCTGATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACG  >  NC_000913/215650‑215797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑