Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,915,177	(T)6→5	coding (358/699 nt)	proQ ←	RNA chaperone ProQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,915,172	0	T	.	100.0%	128.7 / NA	31	coding (363/699 nt)	proQ	RNA chaperone ProQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (17/14);  total (17/14)

GCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTA  >  NC_000913/1915094‑1915248                                                                               |                                                                             gcgtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGttt                                                                            >  1:342029/1‑81 (MQ=255)  cgtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttt                                                                           <  1:341944/81‑1 (MQ=255)        gtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCtt                                                                    <  1:341945/81‑1 (MQ=255)           gTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCt                                                                 >  2:341990/1‑81 (MQ=255)              ggACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGtt                                                              >  1:342009/1‑81 (MQ=255)              ggACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGtt                                                              <  1:341946/81‑1 (MQ=255)               gACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGtt                                                              >  1:341999/1‑80 (MQ=255)               gACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGtt                                                              >  1:341998/1‑80 (MQ=255)                  gTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGc                                                          <  2:341947/81‑1 (MQ=255)                   tGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCa                                                         >  1:341987/1‑81 (MQ=255)                     gCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCAc                                                        <  1:341948/80‑1 (MQ=255)                        tGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCtg                                                    >  2:341996/1‑81 (MQ=255)                         gCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCtgt                                                   >  1:341986/1‑81 (MQ=255)                                 ggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGaa                                            >  2:341994/1‑80 (MQ=255)                                       gTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACgcgc                                     >  1:342004/1‑81 (MQ=255)                                        tGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCt                                    <  2:341949/81‑1 (MQ=255)                                                   tctcACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGcttct                           <  1:341950/79‑1 (MQ=255)                                                       aCCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAgc                     <  1:341951/81‑1 (MQ=255)                                                        ccAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAgct                    >  1:342011/1‑81 (MQ=255)                                                         cagcagTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAgctg                   >  2:342014/1‑81 (MQ=255)                                                          agcagTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAgctgc                  >  2:342013/1‑81 (MQ=255)                                                          agcagTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAgctgc                  <  2:341952/81‑1 (MQ=255)                                                            cagTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTTTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCtt                <  2:341953/81‑1 (MQ=255)                                                               ttGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGc              >  1:342002/1‑80 (MQ=255)                                                               ttGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCg             <  1:341954/81‑1 (MQ=255)                                                               ttGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCg             <  1:341955/81‑1 (MQ=255)                                                                 gCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGa            <  2:341956/80‑1 (MQ=255)                                                                  cGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAg           >  2:342027/1‑80 (MQ=255)                                                                    gCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCAt        <  2:341957/81‑1 (MQ=255)                                                                      aGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGc      >  2:342001/1‑81 (MQ=255)                                                                        cTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGctc    <  1:341958/81‑1 (MQ=255)                                                                         ttCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGctc    >  2:342018/1‑80 (MQ=255)                                                                          tCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTTCACACTCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTa  >  1:342008/1‑81 (MQ=255)                                                                               |                                                                             GCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTA  >  NC_000913/1915094‑1915248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑